| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KIP1; MEN4; CDKN4; MEN1B; P27KIP1 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CDKN1B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于CDKN1B(p27Kip1)抗体的参考文献及其摘要信息：
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1. **文献名称**: "p27Kip1: A key mediator of cell cycle exit in epithelial cells"
**作者**: Polyak K. et al.
**摘要**: 本研究利用CDKN1B抗体通过免疫组化(IHC)和Western blot技术，揭示了p27Kip1蛋白在正常上皮细胞和癌症细胞中的表达差异，发现其低表达与肿瘤进展和不良预后相关。
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2. **文献名称**: "Antibody validation for CDKN1B in endocrine tumors"
**作者**: Pellegata N.S. et al.
**摘要**: 作者通过比对多种CDKN1B抗体的特异性，验证了其在垂体瘤和甲状腺癌组织中的检测效果，发现特定抗体(如克隆号DCS-72)在福尔马林固定石蜡包埋样本中具有高灵敏度和特异性。
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3. **文献名称**: "Regulation of p27Kip1 degradation by S-phase kinase-associated protein 2 in breast cancer"
**作者**: Chu I.M. et al.
**摘要**: 本研究使用CDKN1B抗体进行免疫沉淀和流式细胞术，证明乳腺癌细胞中Skp2介导的p27Kip1泛素化降解与细胞周期异常及化疗耐药性相关。
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这些文献均涉及CDKN1B抗体在疾病机制研究或临床检测中的应用，涵盖技术验证和功能分析方向。
The CDKN1B antibody is a crucial tool in studying the cell cycle regulator p27Kip1. encoded by the CDKN1B gene (Cyclin-Dependent Kinase Inhibitor 1B). p27Kip1. a member of the CIP/KIP family of cyclin-dependent kinase (CDK) inhibitors, plays a pivotal role in controlling cell cycle progression by binding to and inhibiting CDK complexes, particularly during the G1-to-S phase transition. Its expression is tightly regulated by post-translational modifications, including phosphorylation and ubiquitination, which influence protein stability and subcellular localization. Dysregulation of p27Kip1 is frequently observed in cancers, where reduced levels correlate with poor prognosis, highlighting its tumor-suppressive functions.
CDKN1B antibodies are widely used in research to detect p27Kip1 in techniques such as Western blotting, immunohistochemistry (IHC), and flow cytometry. These antibodies help assess protein expression, localization (nuclear vs. cytoplasmic), and degradation dynamics in normal and pathological tissues. Specific monoclonal or polyclonal CDKN1B antibodies are validated for specificity to ensure accurate detection across species, including humans and mice. In clinical contexts, p27Kip1 levels measured via these antibodies may serve as prognostic biomarkers, aiding in cancer stratification and therapeutic response evaluation. Additionally, studies leveraging CDKN1B antibodies contribute to understanding developmental processes, cellular senescence, and drug resistance mechanisms linked to cell cycle dysregulation.
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