| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/400 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RNF58 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DTX2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DTX2抗体的示例参考文献(内容为模拟示例，仅供参考)：
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1. **文献名称**: "DTX2 regulates Notch signaling through ubiquitination of JAGGED1 in triple-negative breast cancer"
**作者**: Chen L, Wang Y, et al.
**摘要**: 本研究利用DTX2特异性抗体，通过免疫共沉淀和Western blot分析，发现DTX2作为E3泛素连接酶介导JAGGED1蛋白的降解，抑制Notch信号通路，从而抑制乳腺癌细胞侵袭。
2. **文献名称**: "Development of a high-affinity monoclonal antibody for DTX2 and its application in colorectal cancer prognosis"
**作者**: Suzuki H, Nakamura K, et al.
**摘要**: 报道了一种新型抗DTX2单克隆抗体的开发，该抗体通过免疫组化验证，可用于结直肠癌组织样本中DTX2蛋白的定量检测，提示DTX2高表达与患者不良预后相关。
3. **文献名称**: "DTX2 promotes genomic instability by inhibiting p53 ubiquitination in ovarian cancer"
**作者**: Gupta R, Patel S, et al.
**摘要**: 研究通过RNA干扰和DTX2抗体功能实验，证明DTX2通过竞争性结合MDM2抑制p53泛素化，导致p53蛋白积累并引发卵巢癌细胞化疗耐药。
4. **文献名称**: "Structural characterization of DTX2 and its interaction with the ESCRT complex"
**作者**: Martinez-Banaclocha M, et al.
**摘要**: 利用抗DTX2抗体进行免疫荧光和质谱分析，揭示了DTX2的C端结构域与ESCRT-III复合物的相互作用，为研究其在细胞膜修复中的作用提供新机制。
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**注意**：以上文献为示例，实际引用时请查询真实数据库(如PubMed、Web of Science)获取准确信息。
The DTX2 antibody targets the Deltex homolog 2 (DTX2) protein, a member of the Deltex family of E3 ubiquitin ligases involved in regulating cellular processes such as Notch signaling, protein degradation, and immune responses. Initially identified as a homolog of Drosophila Deltex, DTX2 plays a role in modulating Notch pathway activity by promoting ubiquitination and degradation of Notch receptors or associated proteins, though its exact mechanisms remain under investigation. Beyond Notch signaling, DTX2 has been implicated in DNA damage repair, apoptosis, and T-cell activation, highlighting its functional diversity.
Antibodies against DTX2 are critical tools for studying its expression patterns, subcellular localization, and interactions in various tissues and disease models. Research has explored DTX2's potential roles in cancer, where dysregulation of ubiquitination pathways often contributes to tumor progression. For instance, DTX2 may act as a tumor suppressor or oncogene depending on context, making it a subject of interest in biomarker and therapeutic discovery. Commercial DTX2 antibodies are typically validated for applications like Western blotting, immunohistochemistry, and co-immunoprecipitation. However, variability in antibody specificity and cross-reactivity with other Deltex family members (e.g., DTX1. DTX3) necessitates careful validation in experimental settings. Ongoing studies aim to clarify DTX2's precise biological functions and its relevance to human diseases.
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