| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1 |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DLK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DLK1抗体的3篇代表性文献示例(注：文献为虚构示例，实际引用需根据真实研究调整)：
1. **文献名称**: "A monoclonal antibody against DLK1 reveals its role in hepatic stem cell differentiation"
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种针对DLK1胞外结构域的单克隆抗体，通过免疫组化和流式细胞术验证其特异性。实验表明，该抗体能有效识别肝癌细胞系和小鼠肝组织中的DLK1蛋白，并证实DLK1在肝干细胞分化中的调控作用。
2. **文献名称**: "DLK1 as a biomarker in adipose tissue: Validation of a polyclonal antibody for ELISA"
**作者**: Chen L, et al.
**摘要**: 研究团队开发了一种兔源多克隆DLK1抗体，并优化其用于血清ELISA检测。结果显示，抗体在肥胖模型小鼠和人类脂肪组织中特异性识别DLK1.证明其可作为代谢疾病研究的可靠工具。
3. **文献名称**: "Targeting DLK1 in neuroendocrine tumors: Immunohistochemical analysis using a novel antibody"
**作者**: García-Ruiz C, et al.
**摘要**: 通过制备高亲和力抗人DLK1抗体，本研究系统评估了DLK1在神经内分泌肿瘤中的表达模式。结果表明，DLK1阳性与肿瘤侵袭性相关，该抗体为临床病理诊断提供了潜在工具。
(提示：实际文献需检索PubMed等数据库，可尝试关键词：DLK1 antibody, DLK1 immunohistochemistry, DLK1 biomarker)
**Background of DLK1 Antibody**
Delta-like homolog 1 (DLK1), also known as preadipocyte factor 1 (Pref-1), is a transmembrane protein belonging to the epidermal growth factor (EGF)-like repeat superfamily. It plays critical roles in cellular differentiation, particularly in adipose tissue, skeletal muscle, and neuroendocrine systems. DLK1 acts as a non-canonical ligand of the Notch signaling pathway, regulating stem cell maintenance, tissue development, and oncogenesis.
DLK1 antibodies are essential tools for detecting and studying the expression, localization, and function of DLK1 in biological systems. These antibodies are widely used in research areas such as cancer biology (e.g., neuroblastoma, hepatocellular carcinoma), metabolic disorders (e.g., obesity, diabetes), and regenerative medicine. DLK1 is often overexpressed in tumors, where it may promote proliferation or inhibit differentiation, making it a potential biomarker or therapeutic target.
Additionally, DLK1 exists in membrane-bound and soluble forms, with the latter generated via proteolytic cleavage. Antibodies specific to different DLK1 isoforms help elucidate their distinct roles in health and disease. Commercial DLK1 antibodies are typically validated for applications like Western blotting, immunohistochemistry, and flow cytometry, with cross-reactivity varying across species (human, mouse, rat). Understanding DLK1's dual roles in development and pathology continues to drive interest in its antibody-based investigation.
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