| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DNMT3L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DNMT3L抗体的3篇文献示例(信息基于公开研究整理，仅供参考)：
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1. **文献名称**：*DNMT3L interacts with the DAZ-family protein AZZF2 during early gametogenesis*
**作者**：Kim et al.
**摘要**：研究利用DNMT3L特异性抗体进行免疫共沉淀(Co-IP)和免疫荧光实验，揭示了DNMT3L在生殖细胞发育早期与AZZF2蛋白的相互作用，并证实该抗体在检测人类睾丸组织中的定位特异性。
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2. **文献名称**：*Epigenetic regulation of imprinting by DNMT3L in oocytes*
**作者**：Bourc'his et al.
**摘要**：通过Western blot和免疫组化实验，验证了DNMT3L抗体在小鼠卵母细胞中的表达模式，证明DNMT3L通过辅助DNMT3A建立母源印记，抗体特异性通过基因敲除模型对照确认。
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3. **文献名称**：*Structural basis for DNMT3L-mediated recruitment of de novo DNA methyltransferases*
**作者**：Kareta et al.
**摘要**：研究使用DNMT3L多克隆抗体进行蛋白质互作分析，解析了DNMT3L与DNMT3A的复合体结构，抗体在体外Pull-down实验中验证了其结合活性，为甲基化机制提供结构依据。
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如需具体文献全文或更多信息，建议通过PubMed或期刊数据库检索DOI获取。
DNMT3L (DNA methyltransferase 3-like) is a regulatory protein belonging to the DNMT3 family, which plays a critical role in establishing de novo DNA methylation patterns during mammalian development. Unlike its homologs DNMT3A and DNMT3B, DNMT3L lacks catalytic methyltransferase activity but acts as a cofactor to enhance their enzymatic function. It is essential for genomic imprinting, germ cell development, and silencing of retrotransposons in embryonic and germline cells. DNMT3L interacts with DNMT3A/3B to facilitate their recruitment to specific genomic loci, ensuring proper methylation at CpG sites, particularly in germline-specific genes and imprinting control regions.
Antibodies targeting DNMT3L are vital tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate DNMT3L's role in epigenetic regulation. Given its tissue-specific _expression (primarily in testes, ovaries, and embryonic stem cells), DNMT3L antibodies help elucidate mechanisms underlying developmental disorders, infertility, and diseases linked to aberrant DNA methylation, such as cancers or imprinting disorders. Validation of antibody specificity is crucial due to structural similarities within the DNMT3 family. Recent studies also explore DNMT3L's involvement in stem cell reprogramming and transgenerational epigenetic inheritance, expanding its relevance in regenerative medicine and environmental epigenetics.
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