Rabbit Polyclonal GNAS Antibody

The GNAS antibody targets proteins encoded by the GNAS gene, a complex locus on chromosome 20q13.3. This gene produces multiple transcripts through alternative splicing and imprinting, including the stimulatory G-protein alpha subunit (Gsα), a critical regulator of cAMP signaling. Gsα mediates cellular responses to hormones and neurotransmitters by activating adenylyl cyclase, influencing processes like cell proliferation, differentiation, and ion transport. GNAS mutations or dysregulation are linked to disorders such as McCune-Albright syndrome (somatic activating mutations), pseudohypoparathyroidism (inactivating mutations), and various endocrine tumors. GNAS antibodies are essential tools in research and diagnostics, enabling detection of Gsα expression or aberrant activation in tissues. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study G protein-coupled receptor (GPCR) signaling pathways, tumorigenesis, and imprinting-related diseases. Additionally, these antibodies aid in distinguishing GNAS-driven pathologies from other cAMP pathway abnormalities. Challenges include cross-reactivity due to the gene’s complex transcription variants and tissue-specific imprinting patterns (e.g., paternal silencing in some tissues), necessitating rigorous validation for specificity. Overall, GNAS antibodies are pivotal in unraveling molecular mechanisms of diseases and advancing therapeutic strategies.