| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | NFAT4; NFATX |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NFATC3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NFATC3抗体的示例参考文献(内容为模拟虚构，仅供参考，建议通过学术数据库核实真实文献)：
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1. **"NFATC3 regulates tumor angiogenesis via transcriptional control of VEGF in breast cancer"**
*Authors: Zhang L, et al.*
**摘要**：本研究通过免疫组化及Western blot分析，利用NFATC3特异性抗体发现其在乳腺癌组织中高表达，并证实NFATC3通过结合VEGF启动子促进血管生成，提示其作为治疗靶点的潜力。
2. **"NFATC3 nuclear translocation in T-cell activation: A flow cytometry-based approach"**
*Authors: Rivera M, et al.*
**摘要**：通过流式细胞术和免疫荧光技术结合NFATC3抗体，揭示了T细胞活化过程中NFATC3的核转位动力学及其与IL-2分泌的调控关系，为免疫调节机制提供新见解。
3. **"Targeting NFATC3 in cardiac hypertrophy: Insights from knockout mouse models"**
*Authors: Chen H, et al.*
**摘要**：利用NFATC3抗体检测基因敲除小鼠心脏组织，发现NFATC3缺失可抑制病理性心肌肥厚相关基因(如ANP)的表达，表明其在心脏疾病中的关键作用。
4. **"NFATC3 interacts with HDAC4 to modulate osteoclast differentiation"**
*Authors: Kim S, et al.*
**摘要**：通过Co-IP和ChIP实验结合NFATC3抗体，证明NFATC3与组蛋白去乙酰化酶HDAC4形成复合物，共同调控破骨细胞分化关键基因TRAP的表达。
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建议通过PubMed或Google Scholar搜索关键词“NFATC3 antibody”、“NFATC3 function”或结合具体研究领域(如癌症、免疫、心血管疾病)获取真实文献。
The nuclear factor of activated T cells 3 (NFATC3), a member of the NFAT transcription factor family, plays a critical role in immune response regulation, cell differentiation, and development. NFAT proteins are calcium-sensitive regulators that translocate to the nucleus upon dephosphorylation by calcineurin, enabling transcriptional activation of target genes. NFATC3. encoded by the *NFATC3* gene, is expressed in immune cells (e.g., T cells), muscle tissues, and the nervous system. It regulates cytokine production, vascular development, and cardiac hypertrophy, and has been implicated in pathological conditions, including cancer, autoimmune diseases, and cardiovascular disorders. Unlike other NFAT isoforms, NFATC3 may exhibit distinct roles in cellular stress responses and oncogenic signaling pathways.
NFATC3 antibodies are essential tools for detecting and quantifying NFATC3 protein expression, localization, and post-translational modifications. These antibodies are widely used in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry to study NFATC3's dynamic shuttling between the cytoplasm and nucleus, its interaction partners, and its regulatory mechanisms. Specific NFATC3 antibodies help differentiate it from other NFAT family members (e.g., NFATC1. NFATC2) due to isoform-specific epitopes. Validation often includes knockout cell lines or siRNA knockdown to confirm specificity. Researchers utilize these antibodies to explore NFATC3's dual roles in immune regulation and disease pathogenesis, particularly its context-dependent pro- or anti-tumor effects and contributions to inflammatory signaling. Understanding NFATC3 dynamics aids in developing therapeutic strategies targeting calcineurin-NFAT pathways.
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