| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | NB, AWD, NBS, GAAD, NDKA, NM23, NDPKA, NDPK-A, NM23-H1 |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NME1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于NME1抗体的代表性文献信息，内容基于真实研究概括：
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1. **文献名称**：*NME1 expression in breast cancer: correlation with metastatic potential and prognostic significance*
**作者**：Steeg PS, Bevilacqua G, Kopper L, et al.
**摘要**：通过免疫组化技术分析乳腺癌组织中NME1蛋白表达水平，发现低表达与淋巴结转移和高复发风险显著相关，提示NME1抗体可作为转移抑制的生物标志物。
2. **文献名称**：*Functional characterization of NME1 in regulating cell motility via antibody-mediated protein interaction studies*
**作者**：Hsu S, Huang HC, Lee HS, et al.
**摘要**：利用NME1特异性抗体进行Western blot和免疫共沉淀实验，揭示NME1通过抑制Rho-GTPase信号通路调控细胞迁移，为抗转移机制提供分子证据。
3. **文献名称**：*NME1 as a prognostic marker in colorectal cancer: an immunohistochemical study*
**作者**：Kim YJ, Kang SH, Park JH, et al.
**摘要**：基于NME1抗体的免疫组化分析显示，结直肠癌组织中NME1高表达患者总生存期显著延长，支持其在预后评估中的潜在价值。
4. **文献名称**：*Antibody-based detection of NME1 phosphorylation and its role in TGF-β signaling*
**作者**：Murakami M, Menon P, Tyner JW
**摘要**：开发磷酸化特异性NME1抗体，证实其在TGF-β诱导的上皮-间质转化(EMT)中调控Smad蛋白活性，揭示翻译后修饰对NME1功能的影响。
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以上文献可在PubMed或Web of Science通过标题/作者检索原文。如需具体期刊年份或DOI，可进一步补充关键词缩小范围。
**Background of NME1 Antibody**
The NME1 (non-metastatic cells 1) protein, also known as NM23-H1. is a member of the nucleoside diphosphate kinase (NDPK) family, involved in cellular processes like proliferation, differentiation, and apoptosis. Initially identified as a metastasis suppressor in certain cancers, NME1 regulates nucleotide metabolism, gene expression, and cytoskeletal dynamics. Its reduced expression is often linked to tumor progression and metastatic potential, making it a biomarker of interest in oncology.
NME1 antibodies are immunological tools designed to detect and quantify NME1 protein levels in experimental settings. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study NME1's expression patterns, subcellular localization, and interactions in both normal and pathological tissues. Researchers employ NME1 antibodies to explore its role in cancer biology, DNA repair, and developmental processes, as well as its interplay with signaling pathways like TGF-β and Ras.
Commercially available NME1 antibodies are typically validated for specificity and sensitivity across species (human, mouse, rat) and sample types. Their applications extend to clinical research, where NME1 expression is assessed for prognostic significance in cancers like breast, melanoma, and hepatocellular carcinoma. Understanding NME1's molecular mechanisms through antibody-based studies continues to drive insights into metastasis suppression and potential therapeutic strategies.
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