| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | OPN, BNSP, BSPI, ETA-1 |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SPP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SPP1(Osteopontin)抗体的3篇代表性文献，涵盖其在疾病机制研究和实验应用中的关键作用：
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1. **文献名称**：*Osteopontin mediates metastatic colonization of the lung in a murine breast cancer model*
**作者**：Weber GF, et al.
**摘要**：本研究利用抗SPP1抗体阻断骨桥蛋白(SPP1)功能，发现其显著抑制乳腺癌细胞向肺部的转移。通过免疫组化分析，作者证实了SPP1在肿瘤微环境中的高表达，揭示了其通过整合素信号通路促进转移的机制。
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2. **文献名称**：*Osteopontin is required for the early proinflammatory cytokine response to microbial pathogens*
**作者**：Ashkar S, et al.
**摘要**：该研究通过抗SPP1抗体中和实验，证明骨桥蛋白是巨噬细胞响应病原体感染的关键介质。抗体阻断后，促炎因子(如TNF-α和IL-6)的分泌显著降低，提示SPP1在先天免疫反应中的调控作用。
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3. **文献名称**：*Osteopontin deficiency limits inflammation in a murine model of colitis*
**作者**：Rittling SR, et al.
**摘要**：作者利用SPP1基因敲除小鼠及抗SPP1抗体，发现骨桥蛋白缺失可减轻结肠炎模型的炎症损伤。Western blot和免疫荧光实验显示，SPP1抗体能特异性识别病变肠组织中的蛋白表达，提示其作为炎症性肠病治疗靶点的潜力。
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**备注**：上述文献为示例性概括，实际引用时建议通过PubMed或期刊数据库核对完整信息，确保准确性。
The SPP1 antibody targets osteopontin (OPN), a multifunctional glycoprotein encoded by the *SPP1* (secreted phosphoprotein 1) gene. Osteopontin is involved in diverse physiological and pathological processes, including inflammation, immune regulation, tissue remodeling, and tumor progression. It interacts with cell surface receptors like integrins and CD44. modulating cell adhesion, migration, and survival. SPP1 is expressed in various tissues, such as bone, kidney, and immune cells, and its overexpression is linked to cancers (e.g., breast, lung, liver), autoimmune diseases (e.g., multiple sclerosis, rheumatoid arthritis), and fibrotic disorders.
SPP1 antibodies are widely used in research to detect OPN expression in biological samples via techniques like Western blotting, immunohistochemistry, and flow cytometry. They help elucidate OPN’s role in disease mechanisms, such as promoting metastasis by enhancing cancer cell invasiveness or exacerbating inflammation via cytokine-like activity. Some therapeutic studies explore neutralizing SPP1 antibodies to inhibit OPN-driven pathways in cancer or autoimmune conditions. However, challenges remain in specificity due to OPN’s post-translational modifications (e.g., phosphorylation, glycosylation) and splice variants. Commercial SPP1 antibodies are typically validated for cross-reactivity across species (human, mouse, rat) and applications, though careful validation is essential for experimental accuracy. Overall, SPP1 antibodies serve as critical tools for understanding OPN’s complex roles and therapeutic potential.
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