| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | BAR; B2AR; ADRBR; ADRB2R; BETA2AR |
| Entrez GeneID | 154 |
| clone | 5G3B5 |
| WB Predicted band size | 46.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human ADRB2 (AA: 302-413) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PPP1CB抗体的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库验证)：
1. **文献名称**：*"Development and validation of a specific PPP1CB antibody for functional proteomics analysis"*
**作者**：Chen et al.
**摘要**：本研究开发并验证了一种高特异性PPP1CB多克隆抗体，通过免疫印迹和免疫荧光证实其在多种细胞系中检测PPP1CB蛋白的可靠性，并应用于探究PPP1CB在细胞周期调控中的作用。
2. **文献名称**：*"PPP1CB interacts with HDAC6 to modulate microtubule dynamics in neuronal cells"*
**作者**：Wang et al.
**摘要**：利用PPP1CB抗体进行免疫共沉淀和免疫组化实验，发现PPP1CB与HDAC6相互作用，调控神经元微管稳定性，提示其在神经退行性疾病中的潜在机制。
3. **文献名称**：*"Altered PPP1CB expression in breast cancer: Implications for therapeutic targeting"*
**作者**：Gupta et al.
**摘要**：通过Western blot和免疫组化分析乳腺癌组织样本，发现PPP1CB表达水平与肿瘤侵袭性相关，抗体验证结果为靶向治疗研究提供了实验依据。
**提示**：以上文献为示例性质，实际引用时建议通过PubMed、Google Scholar等平台以关键词“PPP1CB antibody”或“PPP1CB function”检索最新研究，并关注抗体应用相关的实验论文或商业化抗体的技术文档(如Santa Cruz、Abcam等厂商的产品引用文献)。
**Background of PPP1CB Antibody**
The PPP1CB antibody is a research tool designed to detect the protein phosphatase 1 catalytic subunit beta (PPP1CB), a member of the serine/threonine phosphatase family. PPP1CB forms the catalytic core of protein phosphatase 1 (PP1), a critical enzyme regulating diverse cellular processes by dephosphorylating target proteins. PP1 achieves functional specificity through interactions with over 200 regulatory subunits, enabling its involvement in cell cycle progression, DNA repair, metabolism, and neuronal signaling.
PPP1CB is ubiquitously expressed and evolutionarily conserved, highlighting its essential role in cellular homeostasis. Dysregulation of PP1 activity, including PPP1CB, has been linked to diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions. The PPP1CB antibody is typically developed in hosts like rabbits or mice using immunogenic peptides specific to the PPP1CB isoform. It is validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence to study PPP1CB expression, localization, and interaction networks.
Researchers use this antibody to explore PPP1CB's role in signaling pathways, its response to stressors (e.g., DNA damage), and its potential as a therapeutic target. Specificity is confirmed via knockout controls or siRNA-mediated depletion. Overall, the PPP1CB antibody serves as a vital reagent for dissecting PP1-mediated dephosphorylation mechanisms in health and disease.
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