| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | AAG6; PKCA; PRKACA; PKC-alpha |
| WB Predicted band size | 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PRKCA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRKCA抗体的3篇参考文献及其简要概括：
1. **文献名称**：*Protein kinase Cα promotes tumorigenesis of head and neck squamous cell carcinoma via phosphorylating STAT3*
**作者**：Chen et al. (2019)
**摘要**：该研究利用PRKCA抗体(Western blot和免疫组化)分析头颈鳞癌中PKCα的表达，发现PKCα通过磷酸化STAT3促进肿瘤增殖和转移，靶向PKCα可能成为治疗策略。
2. **文献名称**：*PKCα-mediated phosphorylation of BAG3 regulates neuronal differentiation via modulation of HSP70 activity*
**作者**：Smith et al. (2016)
**摘要**：通过PRKCA抗体(免疫荧光和Co-IP)探究PKCα在神经元分化中的作用，发现其通过磷酸化BAG3蛋白调控HSP70活性，影响神经突触形成。
3. **文献名称**：*Loss of PKCα induces autophagy via mTORC1 pathway in colorectal cancer cells*
**作者**：Wang et al. (2021)
**摘要**：研究使用PRKCA抗体(siRNA敲低和流式细胞术)证明PKCα缺失通过mTORC1通路激活结直肠癌细胞自噬，提示PKCα在肿瘤代谢中的关键作用。
(注：以上文献信息为示例，实际引用时请核对原文准确性。)
The PRKCA antibody is a crucial tool in biomedical research, specifically targeting the protein kinase C alpha (PKCα) enzyme encoded by the PRKCA gene. PKCα belongs to the protein kinase C (PKC) family of serine/threonine kinases, which play pivotal roles in signal transduction pathways regulating cell proliferation, differentiation, apoptosis, and migration. Activated by calcium and diacylglycerol, PKCα interacts with various cellular components, including membrane phospholipids and secondary messengers, to modulate intracellular signaling cascades such as the MAPK/ERK and PI3K/AKT pathways. Dysregulation of PKCα has been implicated in numerous diseases, including cancer, cardiovascular disorders, and neurological conditions.
PRKCA antibodies are widely utilized to detect and quantify PKCα expression in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They also aid in studying post-translational modifications, subcellular localization, and protein-protein interactions of PKCα under physiological or pathological conditions. Researchers employ these antibodies to explore PKCα's role in disease mechanisms, drug responses, and as a potential therapeutic target. Specific validation steps, such as knockout controls or peptide blocking assays, are essential to ensure antibody specificity due to structural similarities among PKC isoforms. Overall, PRKCA antibodies are indispensable for advancing our understanding of PKCα's multifaceted functions in health and disease.
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