| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HIWI2, MIWI2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PIWIL4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PIWIL4抗体相关的文献示例(信息为模拟概括，非真实文献)：
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1. **文献名称**: *PIWIL4 Expression in Human Cancers: A Tissue Microarray Study*
**作者**: Smith J, et al.
**摘要**: 本研究通过免疫组化技术，使用特异性PIWIL4抗体分析其在多种癌症组织中的表达。结果显示，PIWIL4在睾丸癌和卵巢癌中高表达，并与患者预后不良相关。
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2. **文献名称**: *Role of PIWIL4 in Germ Cell Development Revealed by Antibody-Based Localization*
**作者**: Lee S, et al.
**摘要**: 利用PIWIL4特异性抗体进行Western blot和免疫荧光染色，发现PIWIL4蛋白在小鼠睾丸生殖细胞中特异性定位，提示其在精子发生过程中的潜在调控作用。
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3. **文献名称**: *PIWIL4 Antibody Validation for Epigenetic Regulation Studies*
**作者**: Chen R, et al.
**摘要**: 研究验证了商业化PIWIL4抗体的特异性，并发现PIWIL4通过与piRNA复合物相互作用，参与癌症细胞中DNA甲基化修饰的调控。
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注：以上为示例性内容，实际文献需通过PubMed或Google Scholar检索关键词“PIWIL4 antibody”获取。
The PIWIL4 antibody is a crucial tool for studying the PIWI-like protein 4 (PIWIL4), a member of the PIWI/Argonaute protein family involved in germline development, stem cell regulation, and epigenetic silencing via interactions with piwi-interacting RNAs (piRNAs). PIWIL4. also known as HIWI2. is primarily expressed in germline cells and plays roles in gametogenesis, genome stability, and transposon suppression. Unlike other PIWI proteins (PIWIL1-3), PIWIL4 exhibits distinct expression patterns and functions, with emerging roles in somatic cancers, including germ cell tumors, breast cancer, and hepatocellular carcinoma, where its aberrant expression correlates with tumorigenesis and poor prognosis.
PIWIL4 antibodies are designed to detect specific epitopes of the protein, enabling researchers to investigate its expression, localization, and molecular interactions. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to explore PIWIL4's involvement in cellular processes such as cell proliferation, differentiation, and RNA-mediated gene regulation. Recent studies also highlight its potential as a biomarker for cancer diagnosis or therapeutic targeting.
However, challenges remain, including ensuring antibody specificity due to structural similarities among PIWI family members and optimizing detection in diverse sample types. Ongoing research focuses on elucidating PIWIL4's mechanisms in both physiological and pathological contexts, driving demand for high-quality, validated antibodies to advance reproductive biology and oncology studies.
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