| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PEK; WRS; PERK |
| WB Predicted band size | 125 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human EIF2AK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于EIF2AK3抗体的参考文献及其摘要概括：
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1. **文献名称**: "Mutations in the EIF2AK3 gene are associated with Wolcott-Rallison syndrome"
**作者**: Delepine, M., et al.
**摘要**: 该研究首次报道了EIF2AK3基因突变与沃尔科特-拉利森综合征(WRS)的关联，并通过特异性抗体检测患者细胞中EIF2AK3蛋白的表达缺失，揭示了其在胰岛素分泌细胞中的功能重要性。
2. **文献名称**: "Endoplasmic reticulum stress and the role of EIF2AK3 in beta-cell dysfunction"
**作者**: Zhang, P., et al.
**摘要**: 利用EIF2AK3特异性抗体，研究发现内质网应激通过激活EIF2AK3(PERK)通路导致β细胞凋亡，揭示了其在糖尿病发病机制中的作用，并验证了抗体在Western blot和免疫荧光中的应用。
3. **文献名称**: "Antibody-based detection of PERK signaling in neurodegenerative disease models"
**作者**: Unterberger, U., et al.
**摘要**: 通过商业化EIF2AK3抗体，研究分析了阿尔茨海默病模型中PERK通路的激活状态，证实其与神经元内质网应激和tau蛋白病理的相关性，为神经退行性疾病提供了新治疗靶点。
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注：若需具体文献，建议通过PubMed或Google Scholar以关键词“EIF2AK3 antibody”或“PERK antibody”检索近年研究。部分早期经典文献可能未直接使用抗体但涉及功能机制。
The EIF2AK3 antibody targets the protein EIF2AK3 (Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3), also known as PERK (PKR-like ER kinase). As a key sensor in the unfolded protein response (UPR), PERK is activated during endoplasmic reticulum (ER) stress. It phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2α), temporarily halting global protein synthesis to reduce ER workload while promoting selective translation of stress-related genes. This mechanism aids cellular recovery, but chronic activation can trigger apoptosis, linking EIF2AK3 dysfunction to diseases like diabetes, neurodegenerative disorders, and cancer. Mutations in the EIF2AK3 gene cause Wolcott-Rallison syndrome, a rare autosomal recessive condition characterized by infantile diabetes and multisystem complications.
EIF2AK3 antibodies are essential tools for studying PERK expression, activation (e.g., phosphorylated forms), and localization in cells or tissues. They enable detection via Western blotting, immunofluorescence, and immunohistochemistry, facilitating research into ER stress pathways and disease mechanisms. Commercial EIF2AK3 antibodies are widely used in both basic and translational studies to explore therapeutic targets for conditions involving UPR dysregulation. Validation of antibody specificity remains critical, as cross-reactivity with other eIF2α kinases (e.g., GCN2. PKR) may occur. Overall, these antibodies contribute significantly to understanding cellular stress responses and their pathological implications.
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