Human low density lipoprotein receptor,LDLR ELISA Kit

中文名： 人低密度脂蛋白受体(LDLR)ELISA Kit

货号: IPD-E08950h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人低密度脂蛋白受体(LDLR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LDLR含量。LDLR（低密度脂蛋白受体）是调节胆固醇代谢的关键蛋白，其表达受到精确的转录、转录后和翻译后调控。LDLR通过内吞作用清除血液中的LDL，维持胆固醇稳态。研究显示，LDLR的表达和功能受多种因素影响，如胆固醇水平、PCSK9蛋白等，其异常与多种疾病相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.8 pg/mL。本产品适用于科研领域精准检测血清、血浆或组织匀浆样本中LDLR的蛋白表达水平，可为心血管疾病机制研究及药物开发提供实验工具，适用于实验室开展血脂调控通路研究、疾病模型构建或生物标志物筛选等科研项目。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
FH ELISA Kit; FHC ELISA Kit; LDL R ELISA Kit; LDL receptor ELISA Kit; LDLCQ2 ELISA Kit; Ldlr ELISA Kit; LDLR_HUMAN ELISA Kit; Low Density Lipoprotein Receptor ELISA Kit; Low density lipoprotein receptor class A domain containing protein 3 ELISA Kit; Low density lipoprotein receptor familial hypercholesterolemia ELISA Kit; Low-density lipoprotein receptor ELISA Kit
缩写：
LDLR
Uniprot No.：
P01130
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/mL-2000 pg/mL
灵敏度：
7.8 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)223.147228.145235.557220.006229.982237.908SD0.0380.0410.0480.0430.0510.058CV(%)4.5514.8465.5685.19366.69

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LDLR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:20

Average %

Range %

88-99

1:40

Average %

101

Range %

94-105

1:80

Average %

Range %

84-96

1:160

Average %

104

Range %

94-107

回收率：

The recovery of human LDLR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	108	101-112
Heparin plasma (n=4)	94	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.













pg/ml	OD1	OD2	Average	Corrected
2000	2.372	2.3	2.336	2.232
1000	1.738	1.683	1.711	1.607
500	1.32	1.302	1.311	1.207
250	0.818	0.834	0.826	0.722
125	0.552	0.543	0.548	0.444
62.5	0.327	0.317	0.322	0.218
31.25	0.22	0.212	0.216	0.112
0	0.103	0.105	0.104