首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠单核细胞趋化蛋白5(MCP-5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MCP-5含量。MCP - 5是一种关键靶点。它在免疫调节等生理过程中发挥重要作用，可能参与炎症反应等病理进程。目前研究聚焦于其在疾病发生发展中的机制，了解它与免疫细胞的相互作用、信号传导通路等，为开发针对性治疗方法提供依据。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为16.82 pg/mL。适用于科研场景下对小鼠炎症相关模型、免疫调节机制或药物干预效果的研究，例如通过检测血清中MCP - 5水平评估炎症程度，或分析组织匀浆中趋化因子表达变化以探究疾病发生机制，为免疫学、药理学及病理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C-C motif chemokine 12 ELISA Kit; Ccl12 ELISA Kit; CCL12_MOUSE ELISA Kit; Macrophage/monocyte Chemotactic Protein ELISA Kit; MCP-1-related chemokine ELISA Kit; MCP-5 ELISA Kit; Monocyte chemoattractant protein 5 ELISA Kit; Monocyte chemotactic protein 5 ELISA Kit; OTTMUSP00000000792 ELISA Kit; Scya12 ELISA Kit; Small inducible cytokine A12 ELISA Kit; Small-inducible cytokine A12 ELISA Kit
缩写：MCP-5
Uniprot No.：Q62401
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：16.82 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:8 | Average % | 110 | |||||
| Range % | 104-114 | ||||||
回收率：
| The recovery of mouse MCP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-99 | |||||
| EDTA plasma (n=4) | 103 | 98-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.6152.6342.6252.499 5002.1772.0122.0951.969 2501.4321.5021.4671.341 1250.8420.8390.8410.715 62.50.5310.5290.5300.404 31.20.3340.3260.3300.204 15.60.2460.2510.2490.123 00.1250.1270.126 | |||||||
货期：3-5 working days
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