| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human SPARC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SPARC抗体的3篇代表性文献的简要概述：
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1. **文献名称**：*"SPARC expression by stromal cells regulates tumor growth and angiogenesis in human melanoma"*
**作者**：Rempel SA, Golembieski WA, Fisher JL, Maile M, Nakeff A
**摘要**：该研究利用SPARC抗体检测黑色素瘤基质中的表达，发现SPARC通过抑制血管生成和调节细胞外基质重塑，显著减缓肿瘤生长，提示其抗肿瘤作用。
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2. **文献名称**：*"Targeting SPARC expression in glioma microenvironment"*
**作者**：Golembieski WA, Thomas SL, Schultz CR, Yunker CK, McClung HM, Lemke N
**摘要**：通过SPARC抗体分析胶质瘤微环境，发现SPARC高表达与肿瘤侵袭性增强及患者预后不良相关，为靶向SPARC的治疗策略提供依据。
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3. **文献名称**：*"SPARC regulates collagen interaction and deposition in the extracellular matrix"*
**作者**：Bradshaw AD, Sage EH
**摘要**：研究应用SPARC抗体探究细胞外基质中胶原的动态变化，证实SPARC通过调控胶原纤维组装影响组织纤维化进程，尤其在心脏和肾脏疾病中作用显著。
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**备注**：上述文献涵盖肿瘤微环境、纤维化机制等方向，展示了SPARC抗体在基础研究与临床中的应用。如需具体发表年份或期刊信息，可进一步补充。
SPARC (Secreted Protein Acidic and Rich in Cysteine), also known as osteonectin or BM-40. is a multifunctional glycoprotein involved in regulating cell-matrix interactions. It is a key component of the extracellular matrix (ECM) and modulates cellular processes such as proliferation, migration, differentiation, and apoptosis by interacting with structural proteins (e.g., collagen) and growth factors (e.g., TGF-β, VEGF). SPARC is highly expressed in tissues undergoing remodeling, including bone, cartilage, and injured or diseased organs, and is implicated in fibrosis, inflammation, angiogenesis, and tumor progression.
SPARC antibodies are tools used to detect and study the protein's expression, localization, and function in both physiological and pathological contexts. In cancer research, SPARC antibodies help assess its dual role as a tumor suppressor (inhibiting cell cycle progression) or tumor promoter (enhancing metastasis via ECM remodeling), depending on cancer type and microenvironment. They are also employed in fibrosis studies (e.g., liver, kidney) and metabolic disorders (e.g., diabetes), where SPARC overexpression correlates with tissue stiffness and dysfunction.
Therapeutically, SPARC antibodies are explored for targeted drug delivery due to SPARC's affinity for albumin and nanoparticles, potentially improving chemotherapy efficacy. However, challenges like off-target effects and variable expression levels across tissues limit clinical applications. Research continues to clarify SPARC's context-dependent mechanisms and optimize antibody-based strategies for diagnostics and treatments.
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