| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | thp1 |
| Uniprot No | P10070 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 412-641aa |
| 氨基酸序列 | EQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCL |
| 预测分子量 | 28.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于THP1细胞系中重组蛋白研究的3篇文献示例(注：内容为模拟虚构，仅供参考研究方向)：
1. **文献名称**：*Recombinant NLRP3 expression in THP1 cells reveals mechanisms of inflammasome activation*
**作者**：Smith A, et al.
**摘要**：研究通过基因工程在THP1细胞中表达重组NLRP3蛋白，证实其与ASC和Caspase-1的相互作用，揭示了ATP和尿酸结晶激活炎症小体的分子通路。
2. **文献名称**：*Production of recombinant cytokines in differentiated THP1 macrophages for immunotherapy screening*
**作者**：Li X, et al.
**摘要**：利用PMA诱导THP1细胞分化为巨噬细胞后，表达重组IL-1β和TNF-α蛋白，评估其在肿瘤免疫治疗模型中的促炎效应及药物响应。
3. **文献名称**：*CRISPR/Cas9-mediated knock-in of fluorescent-tagged recombinant proteins in THP1 monocytes*
**作者**：Chen R, et al.
**摘要**：通过CRISPR技术在THP1细胞中敲入荧光标记的重组NF-κB蛋白，实时监测LPS刺激下该信号通路的核转位动态及药物抑制作用。
研究方向可涵盖：炎症机制、蛋白功能分析、药物筛选工具开发等。实际文献建议通过PubMed/Google Scholar以“THP1 recombinant protein”等关键词检索。
The THP-1 cell line, derived from acute monocytic leukemia, is a widely used human monocytic model for studying immune responses, inflammation, and cancer. As a suspension cell line, it differentiates into macrophage-like adherent cells upon stimulation with phorbol esters (e.g., PMA), making it valuable for mimicking physiological processes. THP-1 recombinant proteins typically refer to engineered proteins produced using this cell line through genetic modification techniques like plasmid transfection or viral vector transduction.
These recombinant proteins are generated by inserting target genes into THP-1 cells to overexpress specific proteins (e.g., cytokines, receptors, or signaling molecules) or produce modified versions with tags (e.g., GFP, His-tag) for detection and purification. This system enables researchers to investigate protein function, cell signaling pathways, and host-pathogen interactions in a human-relevant context. Common applications include studying NLRP3 inflammasome activation, Toll-like receptor signaling, and macrophage polarization.
The use of THP-1-derived recombinant proteins offers advantages over bacterial expression systems, including proper eukaryotic post-translational modifications and functional protein folding. However, challenges remain in achieving high-yield production due to the cell line's relatively low transfection efficiency compared to engineered CHO or HEK293 cells. Recent advances in CRISPR/Cas9 gene editing and lentiviral transduction have improved recombinant protein yield and stability in THP-1 systems. These tools are particularly valuable for modeling disease-associated mutations and validating drug targets in hematological malignancies or chronic inflammatory conditions.
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