| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik |
| WB Predicted band size | 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NAMPT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3-4条关于NAMPT抗体的参考文献及其简要摘要：
1. **"Structure and function of nicotinamide phosphoribosyltransferase (NAMPT) in mammalian cell survival"**
- **作者**: H. Okamoto 等
- **摘要**: 研究通过NAMPT抗体定位了其在细胞内的表达，揭示了NAMPT在NAD+合成及细胞能量代谢中的关键作用，并探讨其与肿瘤细胞存活的关系。
2. **"NAMPT overexpression drives cancer cell growth and is associated with poor prognosis in colorectal cancer"**
- **作者**: J. Revollo 等
- **摘要**: 利用NAMPT抗体检测结直肠癌组织中蛋白水平，发现NAMPT高表达与肿瘤增殖和患者预后不良相关，提示其作为潜在治疗靶点。
3. **"Extracellular NAMPT (eNAMPT) promotes inflammation through immune cell activation"**
- **作者**: A. Garten 等
- **摘要**: 通过抑制性NAMPT抗体阻断分泌型NAMPT(eNAMPT)的功能，发现其参与炎症反应调控，为治疗自身免疫疾病提供新思路。
4. **"NAMPT as a therapeutic target in tumor microenvironment remodeling"**
- **作者**: Y. Yang 等
- **摘要**: 研究使用NAMPT抗体分析肿瘤微环境中的表达模式，揭示了NAMPT通过调节免疫细胞代谢促进肿瘤免疫逃逸的机制。
这些文献涵盖了NAMPT在代谢、癌症及炎症中的功能研究，抗体主要应用于蛋白定位、表达水平检测及功能抑制实验。
**Background of NAMPT Antibodies**
Nicotinamide phosphoribosyltransferase (NAMPT), also known as pre-B-cell colony-enhancing factor (PBEF) or visfatin, is a key enzyme in the nicotinamide adenine dinucleotide (NAD+) biosynthesis pathway. It catalyzes the conversion of nicotinamide into nicotinamide mononucleotide (NMN), a rate-limiting step in NAD+ production. NAD+ is essential for cellular energy metabolism, DNA repair, and signaling processes, linking NAMPT to critical physiological functions such as aging, inflammation, and stress responses.
NAMPT exists in two forms: intracellular (iNAMPT) and extracellular (eNAMPT). While iNAMPT maintains NAD+ homeostasis, eNAMPT acts as a cytokine, influencing inflammation and immune regulation. Dysregulation of NAMPT is associated with various diseases, including cancer, metabolic disorders (e.g., diabetes, obesity), neurodegenerative conditions, and cardiovascular diseases. Elevated NAMPT levels in tumors, for instance, promote cancer cell survival and chemoresistance by sustaining NAD+-dependent pathways.
NAMPT antibodies are vital tools for studying its expression, localization, and function. They enable detection of NAMPT in techniques like Western blotting, immunohistochemistry (IHC), and ELISA, aiding research into its role in disease mechanisms. Additionally, NAMPT inhibitors (e.g., FK866) and therapeutic antibodies are under investigation for targeting NAMPT-overexpressing cancers or inflammatory conditions. However, challenges remain in understanding tissue-specific NAMPT dynamics and balancing therapeutic efficacy with potential side effects.
Overall, NAMPT antibodies are crucial for both basic research and translational studies, offering insights into its dual roles in health and disease.
×
