| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Cathepsin; CTSL; CTSV; CTSK; CTSH;;Cathepsin L/V/K/H |
| WB Predicted band size | Calculated MW: 37,38 kDa ; Observed MW: 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Cathepsin L/V/K/H |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DNTT(末端脱氧核苷酸转移酶)抗体的3篇代表性文献，按研究领域和内容分类整理：
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### 1. **文献名称**：Terminal deoxynucleotidyl transferase in human leukemias and lymphomas
**作者**：Bollum FJ, Brown M
**摘要概括**：
该研究系统分析了DNTT(TdT)在白血病和淋巴瘤中的表达模式，证实其作为未成熟淋巴细胞标志物的特异性。通过抗体检测发现，TdT在急性淋巴细胞白血病(ALL)和淋巴母细胞淋巴瘤中高表达，为临床诊断和分型提供了重要依据。
### 2. **文献名称**：Immunophenotypic detection of TdT in childhood acute myeloid leukemia
**作者**：Pui CH, Behm FG
**摘要概括**：
研究利用单克隆抗体检测儿童急性髓系白血病(AML)中TdT的表达，发现约20%的AML病例存在TdT阳性细胞。这一发现挑战了TdT仅适用于淋巴细胞肿瘤的传统观点，提示其在混合谱系白血病中的潜在作用。
### 3. **文献名称**：TdT expression in thymic epithelial tumors: a marker for medullary differentiation
**作者**：Kornstein MJ, Bonner H
**摘要概括**：
意外发现部分胸腺上皮肿瘤(如胸腺瘤)的肿瘤微环境中存在TdT阳性淋巴细胞浸润。研究提示DNTT抗体染色可用于区分胸腺瘤与其它纵隔肿瘤，并反映肿瘤微环境中的淋巴细胞未成熟状态。
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**补充说明**：
DNTT抗体主要应用于血液肿瘤诊断(如区分ALL与成熟B细胞肿瘤)，近年研究也扩展至：
- 微小残留病灶(MRD)监测
- 免疫缺陷疾病中的淋巴细胞发育评估
- 实体瘤中未成熟淋巴浸润的分析
如需获取具体实验方案或最新研究进展(如多色流式抗体组合)，可进一步说明需求。
**Background of DNTT (TdT) Antibody**
The DNTT (Deoxynucleotidyl Transferase) antibody targets the enzyme terminal deoxynucleotidyl transferase (TdT), a specialized DNA polymerase critical in lymphoid development. TdT is primarily expressed in immature B- and T-cell precursors during early stages of lymphocyte maturation, where it catalyzes the random addition of nucleotides to V(D)J gene segments. This process enhances antigen receptor diversity, a hallmark of adaptive immunity.
In clinical diagnostics, TdT serves as a key marker for identifying lymphoblastic malignancies. Its expression is strongly associated with acute lymphoblastic leukemia (ALL) and lymphoblastic lymphoma, distinguishing these from mature lymphoid neoplasms or non-hematopoietic tumors. DNTT antibodies are widely used in immunohistochemistry (IHC) and flow cytometry to detect TdT protein in tissue samples or bone marrow aspirates, aiding in disease classification and treatment planning.
Notably, TdT is absent in normal mature lymphocytes, making it a reliable indicator of precursor cell origin. However, its expression may occasionally occur in non-lymphoid tumors, necessitating correlation with other markers. Research also explores TdT's role in autoimmune disorders and immune repertoire studies, highlighting its broader relevance in immunology and oncology.
Overall, DNTT antibodies are indispensable tools for both research and clinical pathology, bridging insights into lymphocyte biology and cancer diagnostics.
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