关键信息
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基因名
TRPV3
- 应用
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别名
VRL3; Vanilloid receptor-like 3
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q8NET8
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表达区间
Asn671~Val790
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分子量
18kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
TRPV3 (Transient Receptor Potential Vanilloid 3) is a member of the TRP channel family that has garnered significant attention due to its role in sensing temperature and mediating pain perception. It is primarily expressed in keratinocytes, which are the predominant cell type in the epidermis, and is implicated in various physiological processes, including thermosensation, inflammation, and skin-related pathologies. The activation of TRPV3 by heat or specific chemical ligands leads to an influx of cations, triggering cellular responses that can influence pain signaling and sensory processing. Research on recombinant TRPV3 proteins aims to elucidate the structure-function relationships of this channel, providing insights into its ion permeability, regulatory mechanisms, and potential therapeutic targets for pain management and dermatological disorders. Understanding the molecular mechanisms underlying TRPV3 activation and its downstream signaling pathways can pave the way for the development of novel analgesics and treatments for skin diseases, highlighting the importance of TRPV3 in both basic and translational research. This has fueled the demand for recombinant TRPV3 proteins, facilitating studies on its pharmacological properties, interactions with ligands, and potential roles in disease states, thus contributing to a broader understanding of sensory biology and its implications in human health.
