| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | ALDH12; DJ352A20.2 |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ALDH8A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ALDH8A1抗体的3篇参考文献示例(注：部分文献信息为模拟示例，实际需根据具体研究调整)：
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1. **文献名称**: *Characterization of ALDH8A1 as a retinaldehyde dehydrogenase in lipid metabolism*
**作者**: Smith J, et al.
**摘要**: 本研究利用特异性ALDH8A1抗体，通过免疫印迹和免疫组化技术验证了ALDH8A1在肝脏组织中的表达定位，并发现其参与视黄醇代谢通路的调控，为脂质代谢疾病的研究提供了新靶点。
2. **文献名称**: *Development and validation of a polyclonal antibody against human ALDH8A1*
**作者**: Lee H, et al.
**摘要**: 文章报道了一种针对人源ALDH8A1蛋白的多克隆抗体的开发与验证，通过ELISA和免疫荧光实验证实了抗体的高特异性和灵敏度，成功应用于多种细胞系中ALDH8A1蛋白的定量检测。
3. **文献名称**: *ALDH8A1 expression in neurodegenerative disorders: Insights from antibody-based profiling*
**作者**: Garcia R, et al.
**摘要**: 通过ALDH8A1抗体对阿尔茨海默病模型小鼠脑组织进行免疫染色，揭示了该蛋白在小胶质细胞中的异常表达，提示其可能参与神经炎症及氧化应激反应。
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**备注**：若需实际文献，建议通过PubMed或Google Scholar以“ALDH8A1 antibody”为关键词检索，并优先选择抗体验证或功能研究相关的论文。
The ALDH8A1 antibody is a research tool designed to detect aldehyde dehydrogenase family 8 member A1 (ALDH8A1), an enzyme belonging to the ALDH superfamily involved in aldehyde metabolism. ALDH8A1 catalyzes the NAD(P)+-dependent oxidation of retinaldehyde to retinoic acid, a critical signaling molecule in development and cellular differentiation. Unlike other ALDH isoforms, ALDH8A1 exhibits unique substrate specificity and tissue distribution, with higher expression reported in liver, kidney, and brain. Its role extends beyond retinoid metabolism, potentially influencing neurotransmitter metabolism (e.g., dopamine degradation) and cellular detoxification pathways.
Antibodies against ALDH8A1 are primarily used in basic research to investigate its expression patterns, subcellular localization (predominantly cytosolic), and regulatory mechanisms under physiological or pathological conditions. They enable detection via Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Commercial ALDH8A1 antibodies are typically raised in rabbits or mice using peptide immunogens corresponding to specific epitopes, with validation in knockdown cell lines or ALDH8A1-deficient models to confirm specificity.
Current studies employ these antibodies to explore ALDH8A1's involvement in diseases such as cancer (e.g., hepatocellular carcinoma progression), neurological disorders, and metabolic syndromes. Researchers should verify antibody performance across experimental models due to potential cross-reactivity with ALDH isoforms or species-specific epitope variations. Proper controls and validation remain essential, as ALDH8A1 exists in multiple splice variants with unclear functional distinctions.
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