Rabbit Polyclonal ACTN1 Antibody

ACTN1 (alpha-actinin-1) is a cytoskeletal protein belonging to the α-actinin family, which plays a critical role in crosslinking actin filaments and anchoring them to cellular structures. Expressed in non-muscle cells, ACTN1 is involved in maintaining cell shape, adhesion, migration, and signaling by linking the actin cytoskeleton to membrane-associated protein complexes, such as integrins at focal adhesions. ACTN1 antibodies are essential tools for studying its expression, localization, and function in cellular processes. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate ACTN1's role in diseases, including cancer, cardiovascular disorders, and immune dysfunction. For instance, altered ACTN1 expression has been linked to tumor metastasis and platelet abnormalities. Structurally, ACTN1 contains an N-terminal actin-binding domain, central spectrin repeats, and a C-terminal calmodulin-like domain, with specific antibodies often targeting these regions to explore functional domains or post-translational modifications. Due to its involvement in cytoskeletal dynamics, ACTN1 is also studied in contexts like wound healing and immune cell activation. Commercially available ACTN1 antibodies vary in host species, clonality, and applications, requiring validation for specificity across experimental models. Research continues to clarify its regulatory mechanisms and therapeutic potential in cytoskeleton-related pathologies.