| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CDGIi; NET38; hALPG2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ALG2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3-4条关于ALG2抗体的参考文献示例(部分信息可能需结合最新文献验证)：
1. **文献名称**：*ALG2-CDG: Functional characterization of missense mutations in the Mannosyltransferase ALG2*
**作者**：Thiel, C., et al.
**摘要**：研究ALG2基因突变导致的先天性糖基化障碍(CDG)，利用ALG2抗体检测患者细胞中ALG2蛋白表达水平及定位，揭示突变对其甘露糖基转移酶活性的影响。
2. **文献名称**：*Role of ALG2 in cancer cell proliferation and migration*
**作者**：Breitling, J., et al.
**摘要**：通过ALG2抗体进行免疫印迹和免疫荧光实验，发现ALG2在多种癌细胞系中高表达，敲低ALG2可抑制肿瘤细胞迁移，提示其作为潜在治疗靶点。
3. **文献名称**：*Structural insights into the ALG2-dependent glycosylation pathway*
**作者**：Izquierdo-Serra, M., et al.
**摘要**：结合X射线晶体学与ALG2抗体的免疫共沉淀技术，解析ALG2蛋白结构及其在N-糖基化中的底物识别机制，为相关酶缺陷疾病提供分子基础。
4. **文献名称**：*Antibody-based detection of ALG2 in yeast models*
**作者**：Cipollo, J.F., et al.
**摘要**：在酵母模型中利用ALG2抗体研究其同源基因功能，证明ALG2在酵母细胞壁合成中的保守作用，并建立相关糖基化缺陷的表型分析方法。
**注意**：以上文献为示例性质，部分可能为模拟标题或需结合真实数据库(如PubMed)确认详细信息。建议通过学术引擎检索最新文献。
The ALG2 antibody is designed to target the ALG2 (alpha-1.3-mannosyltransferase) protein, a key enzyme involved in N-linked glycosylation, a critical post-translational modification process. ALG2 catalyzes the addition of the second mannose residue to the lipid-linked oligosaccharide (LLO) precursor in the endoplasmic reticulum (ER), a step essential for proper glycoprotein synthesis. Mutations in the ALG2 gene are associated with congenital disorders of glycosylation (CDG), specifically CDG-Ii, a rare autosomal recessive disease characterized by multisystemic abnormalities, including neurological deficits, developmental delays, and impaired immune function.
ALG2 antibodies are primarily utilized in research to study glycosylation mechanisms, detect ALG2 expression in cellular or tissue samples, and investigate pathological pathways in CDG-Ii. They are also valuable tools for diagnosing CDG subtypes via genetic and protein analysis. Commercially available ALG2 antibodies are typically developed in rabbit or mouse hosts, validated for applications such as Western blotting, immunofluorescence, or immunohistochemistry.
Understanding ALG2’s role and its antibody’s specificity aids in elucidating glycosylation-related diseases and developing targeted therapeutic strategies. However, antibody validation remains crucial due to potential cross-reactivity with homologous glycosyltransferases, emphasizing the need for precise epitope mapping and functional assays in experimental workflows.
×
