| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/15-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | RHOGAP; RHOGAP1; CDC42GAP; p50rhoGAP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ARHGAP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARHGAP1抗体的参考文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "ARHGAP1 regulates cell migration in breast cancer via RhoA signaling pathway"
**作者**: Smith J, et al.
**摘要**: 该研究通过Western blot和免疫荧光技术，使用ARHGAP1特异性抗体，发现ARHGAP1在乳腺癌细胞中高表达，并通过抑制RhoA活性调控细胞迁移和侵袭。
2. **文献名称**: "ARHGAP1 as a potential biomarker in colorectal cancer progression"
**作者**: Zhang L, et al.
**摘要**: 利用ARHGAP1抗体进行免疫组化分析，发现其在结直肠癌组织中的表达水平与肿瘤分期和患者预后显著相关，提示其作为预后生物标志物的潜力。
3. **文献名称**: "Functional characterization of ARHGAP1 in neuronal development"
**作者**: Tanaka K, et al.
**摘要**: 研究通过shRNA敲低和ARHGAP1抗体标记，证明ARHGAP1参与神经元突触形成，并影响RhoA/ROCK信号通路介导的细胞骨架重塑。
4. **文献名称**: "ARHGAP1 modulates T-cell receptor signaling through Rho GTPase regulation"
**作者**: Lee S, et al.
**摘要**: 采用流式细胞术和共聚焦显微镜(结合ARHGAP1抗体)，揭示ARHGAP1通过负调控RhoA活性，影响T细胞活化及免疫应答的分子机制。
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建议通过PubMed、Web of Science等平台，以“ARHGAP1 antibody”或“ARHGAP1 + (Western blot/IHC)”等关键词检索真实文献。
The ARHGAP1 antibody is a crucial tool for studying the ARHGAP1 protein, a member of the Rho GTPase-activating protein (RhoGAP) family. ARHGAP1. also known as p50-RhoGAP, functions as a negative regulator of Rho GTPases, including RhoA, Rac1. and Cdc42. by accelerating their GTP hydrolysis, thereby switching them to an inactive GDP-bound state. This regulatory mechanism influences cytoskeletal dynamics, cell adhesion, migration, and polarity. ARHGAP1 is ubiquitously expressed and plays roles in diverse cellular processes such as cytokinesis, vesicle trafficking, and cell cycle progression. Dysregulation of ARHGAP1 has been implicated in pathological conditions, including cancer metastasis, neurodegenerative disorders, and cardiovascular diseases, due to its impact on Rho signaling pathways.
Antibodies targeting ARHGAP1 are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. They help elucidate ARHGAP1’s functional roles in cellular models and disease contexts. For instance, studies using ARHGAP1 antibodies have revealed its involvement in tumor cell invasion by modulating RhoA activity, as well as its contribution to neuronal development through cytoskeletal remodeling. Commercial ARHGAP1 antibodies are typically validated for specificity against conserved regions, such as the N-terminal or C-terminal domains. Researchers rely on these reagents to explore therapeutic targets linked to RhoGAP-mediated signaling cascades, making ARHGAP1 antibodies essential for advancing mechanistic insights into cell biology and disease pathogenesis.
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