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产品描述：植物1,5-二磷酸核酮糖羧化酶／加氧酶（RuBisCO）酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测植物组织样本中的RuBisCO含量。RuBisCO即核酮糖-1,5-二磷酸羧化酶/加氧酶，是植物光合作用中催化二氧化碳固定的关键酶。它在全球碳循环中扮演重要角色。其研究机制主要围绕提高它的羧化活性、降低加氧活性，以增强光合效率和作物产量。试剂盒检测范围为3.12 μg/mL-800 μg/mL，灵敏度为3.12 μg/mL。适用于植物生理学研究中光合作用机制解析、环境胁迫（如高温、干旱）对酶活性影响评估、转基因植物代谢通路改造效果验证等科研场景，为植物抗逆性改良、作物产量提升等研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：RuBisCO
种属：Plant
样本类型：plant tissues
检测范围：3.12 μg/mL-800 μg/mL
灵敏度：3.12 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<10% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<20% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
回收率：
| The recovery of plant RuBisCO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| leaf tissue (n=5) | 95 | 89-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 8000.0470.0500.049 2000.2610.2680.265 500.4670.4730.470 12.50.6700.6820.676 3.121.1391.1431.141 01.8611.9161.889 | ||||||
货期：3-5 working days
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