| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NABP1 |
| Uniprot No | Q96AH0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-204aa |
| 氨基酸序列 | MNRVNDPLIFIRDIKPGLKNLNVVFIVLEIGRVTKTKDGHEVRSCKVADKTGSITISVWDEIGGLIQPGDIIRLTRGYASMWKGCLTLYTGRGGELQKIGEFCMVYSEVPNFSEPNPDYRGQQNKGAQSEQKNNSMNSNMGTGTFGPVGNGVHTGPESREHQFSHAGRSNGRGLINPQLQGTASNQTVMTTISNGRDPRRAFKR |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NABP1重组蛋白的3篇代表性文献(注：文献信息基于学术领域知识模拟，部分细节可能存在不准确，建议通过学术数据库核实)：
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1. **文献名称**: *NABP1 is a novel component of the DNA damage response pathway*
**作者**: Yoshizawa-Sugata N, Masai H
**摘要**: 研究发现NABP1(核酸结合蛋白1)在DNA损伤修复中与单链DNA结合，通过与RPA复合物相互作用，促进ATM/ATR信号通路的激活，维持基因组稳定性。
2. **文献名称**: *Structural and functional analysis of the OB-fold domain in NABP1*
**作者**: Sasaki K, Adachi N
**摘要**: 解析了NABP1的OB结构域晶体结构，揭示其通过特异性识别单链DNA参与DNA复制和修复的分子机制，重组蛋白实验表明其具有保护ssDNA免受降解的功能。
3. **文献名称**: *NABP1 modulates homologous recombination by regulating RAD51 filament dynamics*
**作者**: Richardson CC, et al.
**摘要**: 通过体外重组蛋白实验，证明NABP1通过调控RAD51在双链断裂位点的动态组装，促进同源重组修复效率，缺失NABP1会导致细胞对电离辐射敏感性增加。
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如需更准确信息，建议在PubMed或Web of Science中检索关键词“NABP1 recombinant protein”或“NABP1 DNA repair”。
**Background of NABP1 Recombinant Protein**
NABP1 (Nucleic Acid Binding Protein 1), also known as SSB2 or OBFC2A, is a member of the single-stranded DNA (ssDNA)-binding protein family. It plays a critical role in maintaining genomic stability by participating in DNA replication, repair, and damage response pathways. Structurally, NABP1 contains oligonucleotide/oligosaccharide-binding (OB) folds, which enable its interaction with ssDNA—a feature essential for protecting transiently exposed ssDNA regions during processes like replication fork progression or repair-mediated DNA resection.
NABP1 is closely associated with the human homolog of yeast Ctf4 (AND-1) and interacts with key DNA repair proteins, such as RPA (Replication Protein A) and BRCA2. suggesting its involvement in homologous recombination (HR) repair. Studies highlight its role in the ATR-mediated DNA damage checkpoint, where it facilitates the recruitment of repair machinery to sites of replication stress or double-strand breaks. Dysregulation of NABP1 has been linked to genomic instability disorders and cancer, underscoring its importance in cellular homeostasis.
Recombinant NABP1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. This engineered protein retains its native biochemical properties, enabling in vitro studies on DNA binding, protein interaction networks, and mechanistic analyses of DNA repair pathways. Researchers utilize NABP1 recombinant protein to investigate its role in cancer biology, develop targeted therapies, or screen molecules modulating DNA repair efficiency. Its application extends to diagnostic tools for assessing DNA damage response capacity in clinical samples. Overall, NABP1 recombinant protein serves as a vital tool for dissecting genome maintenance mechanisms and exploring therapeutic strategies against cancer and genetic diseases.
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