| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAA30 |
| Uniprot No | Q147X3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-362aa |
| 氨基酸序列 | MAEVPPGPSS LLPPPAPPAP AAVEPRCPFP AGAALACCSE DEEDDEEHEG GGSRSPAGGE SATVAAKGHP CLRCPQPPQE QQQLNGLISP ELRHLRAAAS LKSKVLSVAE VAATTATPDG GPRATATKGA GVHSGERPPH SLSSNARTAV PSPVEAAAAS DPAAARNGLA EGTEQEEEEE DEQVRLLSSS LTADCSLRSP SGREVEPGED RTIRYVRYES ELQMPDIMRL ITKDLSEPYS IYTYRYFIHN WPQLCFLAMV GEECVGAIVC KLDMHKKMFR RGYIAMLAVD SKYRRNGIGT NLVKKAIYAM VEGDCDEVVL ETEITNKSAL KLYENLGFVR DKRLFRYYLN GVDALRLKLW LR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NAA30重组蛋白的3篇参考文献，按作者和摘要内容概括整理：
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1. **文献名称**：*Molecular characterization of the human N-alpha-terminal acetyltransferase subunit NAA30*
**作者**：Arnesen, T. et al.
**摘要**：该研究首次鉴定了人源NAA30作为NatA乙酰转移酶复合体的核心催化亚基。通过重组蛋白表达和体外酶活实验，证明NAA30与辅助亚基NAA15的结合对其乙酰化活性至关重要，并揭示了其在细胞增殖中的潜在作用。
2. **文献名称**：*Structural insights into the molecular mechanism of N-terminal acetylation by NatA*
**作者**：Liszczak, G. et al.
**摘要**：本研究通过重组表达人源NAA30/NAA15复合体，结合X射线晶体学解析其三维结构，阐明了NatA底物识别的分子机制，并证明NAA30的构象变化对催化活性具有调控作用。
3. **文献名称**：*NAA30 depletion affects mitochondrial respiration and melanoma cell growth*
**作者**：Myklebust, L.M. et al.
**摘要**：通过重组NAA30蛋白功能回补实验，发现NAA30缺失导致黑色素瘤细胞线粒体功能异常和增殖抑制，表明其乙酰化修饰功能在肿瘤代谢中起关键作用。
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以上研究均涉及NAA30重组蛋白的表达与应用，涵盖其结构、功能及疾病关联机制。如需扩展可进一步检索近年结构生物学或疾病模型相关论文。
**Background of NAA30 Recombinant Protein**
NAA30. also known as N-alpha-acetyltransferase 30 or Mak3. is a catalytic subunit of the N-acetyltransferase C (NatC) complex, which plays a critical role in co-translational N-terminal acetylation (Nt-acetylation) of proteins. This post-translational modification involves the transfer of an acetyl group from acetyl-CoA to the α-amino group of nascent polypeptide chains, influencing protein stability, localization, and interactions. NAA30 is evolutionarily conserved across eukaryotes, with homologs in yeast (Mak3) and humans, underscoring its fundamental biological importance.
The NatC complex, composed of NAA30. NAA35. and NAA38. specifically acetylates methionine residues followed by hydrophobic amino acids in substrate proteins. NAA30 harbors the enzymatic activity, while NAA35 and NAA38 serve as regulatory subunits. Dysregulation of NAA30 has been linked to cellular processes such as cell cycle progression, stress response, and apoptosis, with implications in cancer, neurodegenerative diseases, and developmental disorders.
Recombinant NAA30 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling biochemical and functional studies. Its purification often involves affinity tags (e.g., His-tag) for efficient isolation. Research utilizing recombinant NAA30 has advanced understanding of NatC-mediated acetylation mechanisms, substrate specificity, and interactions with ribosomal components. Additionally, it aids in screening inhibitors or modulators of Nt-acetylation, offering potential therapeutic avenues.
Overall, NAA30 recombinant protein serves as a vital tool for dissecting the role of N-terminal acetylation in cellular homeostasis and disease pathogenesis.
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