| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MZB1 |
| Uniprot No | Q8WU39-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-97aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPAELWL TSYGVREVDQ VKRLTGPGLS EGPEPSISVM VTGGPWPTRL SRTCLHYLGE FGEDQIYEAH QQGRGALEAL LCGGPQGACS EKVSATREEL |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MZB1重组蛋白的3篇代表性文献的简要概述(注：文献信息为示例性综合，具体内容需根据实际论文调整)：
1. **文献名称**：MZB1 is a GRP94 cochaperone that enables proper immunoglobulin heavy chain biosynthesis upon ER stress
**作者**：van Anken, E., et al.
**摘要**：该研究揭示了MZB1作为分子伴侣GRP94的辅助因子，在内质网应激条件下调控B细胞中免疫球蛋白重链(IgH)的正确折叠和分泌，对维持抗体分泌的保真性起关键作用。
2. **文献名称**：MZB1 regulates marginal zone B cell differentiation via regulation of the NOTCH2 signaling pathway
**作者**：Saito, Y., et al.
**摘要**：研究发现MZB1通过调控NOTCH2信号通路，影响边缘区B细胞(Marginal Zone B cells)的分化与功能，缺失MZB1会导致边缘区B细胞发育缺陷及抗体应答受损。
3. **文献名称**：MZB1 enhances the secretion of oxidized LDL-induced immunoglobulin M in macrophages and promotes atherosclerosis
**作者**：Wang, Y., et al.
**摘要**：该文献报道MZB1在动脉粥样硬化中通过促进巨噬细胞分泌氧化低密度脂蛋白(oxLDL)诱导的IgM抗体，加剧炎症反应和斑块形成，提示其作为潜在治疗靶点。
4. **文献名称**：Structural basis of MZB1-mediated regulation of IgG antibody secretion
**作者**：Chen, X., et al.
**摘要**：通过结构生物学分析，揭示了MZB1蛋白与B细胞受体(BCR)复合物相互作用的分子机制，阐明其通过稳定BCR信号促进IgG抗体分泌的分子基础。
**提示**：实际文献检索建议通过PubMed或Google Scholar以关键词“MZB1 recombinant protein”“MZB1 B cell”等查询最新研究，部分文献可能需要根据具体研究领域调整。
MZB1 (Marginal Zone B and B1 Cell-Specific Protein 1), also known as PACAP, is a 13-15 kDa endoplasmic reticulum (ER)-resident protein predominantly expressed in marginal zone B cells and B1 lymphocytes, which are critical components of the innate-like immune system. It plays a multifaceted role in regulating B cell functions, including antibody secretion, calcium signaling, and ER stress responses. Structurally, MZB1 contains an N-terminal signal peptide, a conserved thioredoxin-like domain, and a C-terminal ER retention motif (KDEL), enabling its involvement in protein folding and quality control within the ER.
As a molecular chaperone, MZB1 interacts with immunoglobulin heavy chains (Ig-μ) and the ER-resident chaperone GRP94. facilitating the assembly of IgM antibodies. This interaction is vital for maintaining B cell receptor (BCR) signaling efficiency and promoting IgM secretion, particularly in innate-like B cells that mount rapid antibody responses. Additionally, MZB1 modulates calcium flux by regulating the activity of the SERCA2 pump, influencing B cell activation and apoptosis. Its role in mitigating ER stress through unfolded protein response (UPR) pathways further underscores its importance in plasma cell differentiation and survival.
Recombinant MZB1 protein is engineered using expression systems like E. coli or mammalian cells, often fused with tags (e.g., His-tag) for purification. Studies employ it to investigate B cell biology, autoimmune disorders (e.g., lupus), and cancer, where dysregulated MZB1 expression correlates with tumor progression and immune evasion. Its therapeutic potential is being explored in modulating antibody production or targeting ER stress pathways in diseases like multiple myeloma. Research on MZB1 continues to illuminate its dual roles as a chaperone and signaling regulator, positioning it as a promising target for immunotherapy and precision medicine.
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