| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYOZ1 |
| Uniprot No | Q9NP98 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPLSGTP APNKKRKSSK LIMELTGGGQ ESSGLNLGKK ISVPRDVMLE ELSLLTNRGS KMFKLRQMRV EKFIYENHPD VFSDSSMDHF QKFLPTVGGQ LGTAGQGFSY SKSNGRGGSQ AGGSGSAGQY GSDQQHHLGS GSGAGGTGGP AGQAGRGGAA GTAGVGETGS GDQAGGEGKH ITVFKTYISP WERAMGVDPQ QKMELGIDLL AYGAKAELPK YKSFNRTAMP YGGYEKASKR MTFQMPKFDL GPLLSEPLVL YNQNLSNRPS FNRTPIPWLS SGEPVDYNVD IGIPLDGETE EL |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYOZ1重组蛋白研究的示例参考文献(内容为示例，建议通过学术数据库核实具体信息)：
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1. **标题**: *MYOZ1 regulates skeletal muscle calcineurin signaling through interaction with RCAN1*
**作者**: Frey N, et al.
**摘要**: 研究通过重组MYOZ1蛋白发现其与RCAN1(calcineurin抑制剂)相互作用，调控骨骼肌中calcineurin信号通路，可能影响肌肉分化和适应性肥大。
2. **标题**: *Myozenin-1 interacts with γ-filamin to stabilize the Z-disc in striated muscle*
**作者**: Faulkner G, et al.
**摘要**: 利用重组MYOZ1蛋白进行体外结合实验，发现其与γ-filamin直接结合，维持横纹肌Z盘结构稳定性，并可能参与肌节机械应力响应。
3. **标题**: *MYOZ1 mutations and expression in human cardiomyopathy*
**作者**: Hogan KA, et al.
**摘要**: 通过重组MYOZ1蛋白功能分析，揭示其突变与扩张型心肌病的关联，突变体导致心肌细胞中calcineurin信号异常激活。
4. **标题**: *Proteomic analysis of MYOZ1-associated complexes in cardiac muscle*
**作者**: Liu Y, et al.
**摘要**: 基于重组MYOZ1的亲和纯化质谱分析，鉴定其在心肌中与α-actinin-2和MLP等蛋白形成复合物，参与机械-信号转导调控。
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**注意**：以上文献信息为示例，实际研究需以权威数据库(如PubMed、Web of Science)检索结果为准。重组蛋白研究多聚焦于MYOZ1的相互作用网络、信号调控及疾病机制。
**Background of MYOZ1 Recombinant Protein**
MYOZ1 (myozenin 1), also known as calsarcin-2. is a striated muscle-specific protein belonging to the sarcomeric Z-disc-associated myozenin family. It plays a regulatory role in muscle function by interacting with structural and signaling proteins, including α-actinin, γ-filamin, and calcineurin. MYOZ1 is predominantly expressed in skeletal muscle, where it contributes to Z-disc stability and modulates intracellular signaling pathways, particularly the calcineurin/NFAT pathway, which influences muscle fiber-type specification, hypertrophy, and adaptive responses.
Recombinant MYOZ1 protein is engineered through molecular cloning techniques, typically using bacterial or mammalian expression systems to produce purified, biologically active protein. The recombinant form retains functional domains critical for protein-protein interactions, enabling its use in studying MYOZ1’s role in muscle physiology and disease. Researchers employ it to investigate mechanisms underlying muscular dystrophies, cardiomyopathies, and other myopathies linked to Z-disc dysfunction. Additionally, it serves as a tool for identifying binding partners, validating antibody specificity, or screening small molecules targeting MYOZ1-related pathways.
Studies highlight MYOZ1’s dual role as a structural scaffold and signaling modulator, with implications in muscle atrophy, regeneration, and metabolic regulation. Its recombinant variant provides a controlled platform for dissecting these processes *in vitro* or in cell-based assays. Further research aims to explore MYOZ1’s therapeutic potential in muscle-related disorders and its interplay with exercise-induced adaptations.
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