| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYL6B |
| Uniprot No | P14649 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-208aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMPPKKDVPVKKPAGPSISKPAAKPAAA GAPPAKTKAEPAVPQAPQKTQEPPVDLSKVVIEFNKDQLEEFKEAFELFD RVGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDELKSRRVDFETF LPMLQAVAKNRGQGTYEDYLEGFRVFDKEGNGKVMGAELRHVLTTLGEKM TEEEVETVLAGHEDSNGCINYEAFLKHILSV |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYL6B重组蛋白的模拟参考文献示例(请注意，这些为虚构内容，实际文献需通过学术数据库查询)：
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1. **"Functional characterization of recombinant MYL6B in cytoskeletal dynamics"**
*作者：Chen, L., et al. (2020)*
**摘要**：本研究成功表达并纯化了重组MYL6B蛋白，发现其通过调控肌球蛋白II的ATP酶活性参与细胞骨架重组，影响癌细胞迁移和侵袭能力。
2. **"Structural insights into MYL6B and its role in myosin assembly"**
*作者：Wang, Y., et al. (2018)*
**摘要**：利用X射线晶体学解析了重组MYL6B的三维结构，揭示了其与肌球蛋白重链的结合位点，为理解肌肉收缩分子机制提供结构基础。
3. **"Recombinant MYL6B expression in neuronal development"**
*作者：Kim, S., et al. (2019)*
**摘要**：通过体外重组表达MYL6B，证明其在神经元突触形成中通过调节囊泡运输影响突触可塑性，提示其潜在神经发育相关功能。
4. **"MYL6B knockdown and recombinant rescue in cardiac hypertrophy models"**
*作者：Rodriguez, J., et al. (2021)*
**摘要**：构建重组MYL6B蛋白并用于心肌细胞实验，证实其通过钙离子信号通路抑制病理性心肌肥厚，提出其作为心脏疾病治疗靶点。
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**建议**：实际研究中请通过PubMed、Google Scholar或Web of Science等平台，以“MYL6B recombinant protein”或“MYL6B expression and function”为关键词检索最新文献。
**Background of MYL6B Recombinant Protein**
MYL6B (Myosin Light Chain 6B) is a regulatory protein belonging to the myosin light chain family, which plays a critical role in cellular motility, contractility, and intracellular transport. It is a component of myosin II, a motor protein complex essential for actin-based processes such as muscle contraction, cell division, and maintenance of cell shape. MYL6B specifically associates with the myosin heavy chain to modulate ATPase activity, enabling mechanical force generation during these processes.
The gene encoding MYL6B is located on human chromosome 12 (12q13.2) and is ubiquitously expressed across tissues, with higher levels observed in smooth muscle and non-muscle cells. Unlike other myosin light chains, MYL6B is classified as an essential light chain, meaning it is indispensable for the structural stability and functional integrity of the myosin II complex. Dysregulation of MYL6B has been implicated in pathological conditions, including cardiovascular disorders, cancer metastasis, and developmental defects, highlighting its biological significance.
Recombinant MYL6B protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research applications. This recombinant form allows scientists to study MYL6B’s interactions with myosin heavy chains, its role in cytoskeletal dynamics, and its contribution to disease mechanisms. It is also utilized in drug discovery screens targeting myosin-related pathways and in structural studies to elucidate conformational changes during motor activity.
Overall, MYL6B recombinant protein serves as a vital tool for dissecting the molecular basis of cellular mechanics and exploring therapeutic interventions for myosin-linked diseases.
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