| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYL6 |
| Uniprot No | P60660 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-151aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMCDFTEDQTAEFKEAFQLFDRTGDGKILYS QCGDVMRALGQNPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKN KDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEMLVA GHEDSNGCINYEELVRMVLNG |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYL6重组蛋白的模拟参考文献示例，供参考：
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1. **标题**: *"Expression and Purification of Recombinant MYL6 in Escherichia coli for Structural Analysis"*
**作者**: Smith A, et al.
**摘要**: 本研究成功克隆并表达了人源MYL6蛋白于大肠杆菌系统，优化了纯化条件以获得高纯度蛋白。重组MYL6通过镍柱亲和层析和凝胶过滤分离，为后续晶体学研究奠定了基础。
2. **标题**: *"Structural Insights into MYL6-Myosin Heavy Chain Interaction Using Recombinant Proteins"*
**作者**: Johnson B, et al.
**摘要**: 通过重组表达的MYL6蛋白与肌球蛋白重链体外结合实验，揭示了二者结合的关键结构域，并利用表面等离子体共振(SPR)测定了结合亲和力，阐明了其在肌球蛋白复合体组装中的作用机制。
3. **标题**: *"Crystal Structure of MYL6 Reveals Conserved Regulatory Motifs in Myosin Light Chains"*
**作者**: Lee C, et al.
**摘要**: 本研究解析了重组人MYL6蛋白的X射线晶体结构(分辨率2.1Å)，发现其EF-hand钙离子结合基序的构象变化，为理解MYL6在细胞收缩中的调控提供了结构基础。
4. **标题**: *"Functional Rescue of MYL6 Knockdown in Cancer Cell Migration via Recombinant Protein Supplementation"*
**作者**: Wong D, et al.
**摘要**: 在MYL6敲低的肿瘤细胞中，外源添加重组MYL6蛋白可恢复细胞迁移能力，表明其在肿瘤转移中的潜在作用，并验证了重组蛋白在功能恢复实验中的有效性。
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**说明**：上述文献为模拟示例，实际引用时需通过学术数据库(如PubMed、Web of Science)核实真实文献。若需具体文章，建议检索关键词“MYL6 recombinant expression”或“MYL6 structural/functional study”。
**Background of MYL6 Recombinant Protein**
MYL6 (Myosin Light Chain 6) is a component of the myosin complex, a motor protein critical for cellular motility, muscle contraction, and intracellular transport. It belongs to the myosin light chain family, which regulates the structural and functional dynamics of myosin by modulating its ATPase activity and interaction with actin filaments. MYL6 specifically associates with myosin heavy chains in non-muscle cells and certain muscle tissues, playing a role in maintaining cytoskeletal organization, cell shape, and contractile processes.
The recombinant MYL6 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. Its production enables detailed studies of myosin-mediated mechanisms in vitro, such as actomyosin contractility, vesicle trafficking, and cell division. Researchers utilize MYL6 recombinant proteins to investigate its binding partners, phosphorylation events, and regulatory roles in signaling pathways like RhoA/ROCK, which influence cell migration and tissue morphogenesis.
Dysregulation of MYL6 has been implicated in pathological conditions, including cardiovascular diseases, muscular disorders, and cancer metastasis, where aberrant cell motility or contractility drives progression. Recombinant MYL6 serves as a tool for drug screening, structural studies, and functional assays to identify therapeutic targets. Its application extends to developing biosensors for real-time monitoring of cytoskeletal dynamics.
Overall, MYL6 recombinant protein is a vital resource for advancing mechanistic insights into cellular mechanics and disease pathways, bridging molecular biology with translational research.
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