| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYL5 |
| Uniprot No | Q02045 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-132aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMDQNRDGFIDKEDLKDTYASLGKTNV KDDELDAMLKEASGPINFTMFLNLFGEKLSGTDAEETILNAFKMLDPDGK GKINKEYIKRLLMSQADKMTAEEVDQMFQFASIDVAGNLDYKALSYVITH GEEKEE |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于领域相关研究的假设性参考文献示例(注：部分信息为模拟虚构，建议通过学术数据库核实)：
1. **标题**: "Recombinant Expression and Purification of Human MYL5 in E. coli"
**作者**: Chen X, et al.
**摘要**: 报道了利用大肠杆菌系统表达人源MYL5重组蛋白的优化方法，通过His标签纯化获得高纯度蛋白，并验证其与肌球蛋白重链的体外结合活性。
2. **标题**: "MYL5 Regulates Skeletal Muscle Differentiation via Calcium Signaling"
**作者**: Tanaka K, et al.
**摘要**: 发现重组MYL5蛋白在成肌细胞分化中调控钙离子通道活性，通过CRISPR敲除实验证实其影响肌管形成的分子机制。
3. **标题**: "Structural Analysis of MYL5 Reveals Conserved Binding Motifs"
**作者**: Müller R, et al.
**摘要**: 采用X射线晶体学解析了重组MYL5的三维结构，揭示了其与肌球蛋白ATP酶活性区域相互作用的关键氨基酸残基。
4. **标题**: "MYL5 Dysfunction in Cardiomyopathy: Insights from Recombinant Models"
**作者**: Gupta S, et al.
**摘要**: 通过体外重组蛋白实验及小鼠模型，证明MYL5突变导致心肌收缩异常，提示其在遗传性心脏病中的潜在作用。
**建议**：实际文献请通过PubMed或Google Scholar以“MYL5 recombinant”、“Myosin Light Chain 5 expression”等关键词检索，优先选择近五年内发表的实验性研究。
**Background of MYL5 Recombinant Protein**
MYL5. or myosin light chain 5. is a regulatory component of myosin, a motor protein critical for muscle contraction and non-muscle cellular processes such as cell motility, cytokinesis, and intracellular transport. It belongs to the myosin light chain family, which modulates the ATPase activity of myosin heavy chains, enabling mechanical force generation. MYL5 is specifically associated with myosin II isoforms and plays a role in smooth muscle and non-muscle cells.
Recombinant MYL5 protein is engineered through genetic cloning, where the MYL5 gene is expressed in heterologous systems like *E. coli* or mammalian cell lines. This allows large-scale production of purified, functional MYL5 for research and therapeutic applications. The recombinant form retains the ability to bind myosin heavy chains and regulate actin-myosin interactions, making it a valuable tool for studying cytoskeletal dynamics, muscle physiology, and diseases linked to myosin dysfunction, such as cardiovascular disorders, cancer metastasis, and developmental abnormalities.
In research, MYL5 recombinant protein is utilized to investigate signaling pathways involving calcium/calmodulin and Rho kinase, which phosphorylate MYL5 to modulate contractile activity. It also aids in drug discovery targeting myosin-related pathologies. Structural studies using recombinant MYL5 have clarified its role in myosin assembly and mechanochemical coupling. Overall, MYL5 recombinant protein bridges molecular biology and clinical insights, offering a controlled platform to explore cellular mechanics and therapeutic interventions.
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