| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MLCK; MLCK2; caMLCK |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MYLK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MYLK3抗体的模拟参考文献示例(请注意，这些文献为虚构示例，仅供参考)：
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1. **文献名称**: *MYLK3 Antibody Validation in Cardiac Hypertrophy Models*
**作者**: Smith J, et al.
**摘要**: 本研究验证了一种特异性识别MYLK3的多克隆抗体，通过Western blot和免疫组化证实其在心脏组织中的表达。研究发现MYLK3在心肌肥厚模型中表达上调，提示其可能参与病理性心脏重塑的调控。
2. **文献名称**: *Role of MYLK3 in Smooth Muscle Contraction: Antibody-Based Inhibition Studies*
**作者**: Zhang Y, et al.
**摘要**: 利用MYLK3单克隆抗体阻断其激酶活性，发现MYLK3通过磷酸化肌球蛋白轻链调节平滑肌收缩。抗体在功能实验中的应用为MYLK3的机制研究提供了工具支持。
3. **文献名称**: *Development of a High-Affinity MYLK3 Antibody for Cancer Biomarker Screening*
**作者**: Lee H, et al.
**摘要**: 研究团队开发了一种高亲和力MYLK3抗体，用于检测肿瘤组织中的MYLK3表达水平。临床样本分析显示，MYLK3在部分实体瘤中显著高表达，可能与肿瘤侵袭性相关。
4. **文献名称**: *MYLK3 Genetic Variants and Protein Localization: Insights from Immunofluorescence*
**作者**: Garcia R, et al.
**摘要**: 通过MYLK3抗体结合免疫荧光技术，发现MYLK3主要定位于细胞质，其遗传变异可能影响蛋白稳定性及细胞骨架动力学，为相关遗传疾病研究提供依据。
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注：实际研究中建议通过PubMed、Web of Science等平台检索真实文献。若需具体文献协助，请提供更多背景信息。
**Background of MYLK3 Antibody**
MYLK3 (myosin light chain kinase 3) is a member of the myosin light chain kinase family, which regulates smooth muscle and non-muscle cell contraction by phosphorylating myosin regulatory light chains. Unlike other MYLK isoforms (e.g., MYLK1/MYLK2), MYLK3 is predominantly expressed in cardiac and skeletal muscle tissues, where it plays a role in modulating muscle function and stress responses. Studies suggest MYLK3 is involved in cardiac development, contractility, and adaptation to mechanical or pathological stimuli, though its precise mechanisms remain less characterized compared to other family members.
The MYLK3 antibody is a critical tool for detecting and quantifying MYLK3 protein expression in research. It enables investigations into MYLK3's subcellular localization, interaction partners, and phosphorylation dynamics, particularly in cardiac hypertrophy, heart failure, or muscular disorders. Commercial MYLK3 antibodies are typically developed in rabbits or mice using immunogenic peptide sequences unique to MYLK3. ensuring specificity. Validation often includes Western blotting, immunohistochemistry, or immunofluorescence in relevant tissues or cell lines.
Recent interest in MYLK3 stems from its potential role in cardiomyopathies and as a biomarker for cardiac dysfunction. However, functional studies remain limited, underscoring the need for reliable antibodies to advance understanding of MYLK3's physiological and pathological contributions.
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