| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | mATG9; APG9L1; MGD3208 |
| WB Predicted band size | 94 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Fusion protein of human ATG9A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ATG9A抗体的3篇参考文献及其简要摘要：
1. **文献名称**: *Mammalian autophagy: core molecular machinery and signaling regulation*
**作者**: Daniel J. Klionsky, et al.
**摘要**: 该综述系统总结了自噬相关蛋白(包括ATG9A)在哺乳动物细胞中的核心作用，强调ATG9A作为唯一跨膜自噬蛋白的功能，参与自噬体形成初期的膜运输过程，并讨论了其抗体在自噬动态研究中的应用。
2. **文献名称**: *ATG9A shapes the forming autophagosome through Arfaptin 2 and phosphatidylinositol 4-kinase IIIβ*
**作者**: Mariángela Segarra-Mondejar, et al.
**摘要**: 研究通过免疫沉淀和活细胞成像技术，揭示了ATG9A通过招募Arfaptin 2和PI4KIIIβ调控自噬体膜的脂质组成，其特异性抗体被用于追踪ATG9A在细胞内的动态定位及相互作用。
3. **文献名称**: *Structure of the human ATG9A-ATG2A complex reveals critical roles for lipid transport in autophagosome formation*
**作者**: Yasunori Watanabe, et al.
**摘要**: 该研究利用冷冻电镜解析了ATG9A与ATG2A的复合物结构，证明两者协同介导自噬体膜的脂质运输，实验中通过ATG9A抗体验证了其在脂质转移中的关键构象变化。
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如需更具体的信息(如期刊、年份或DOI)，可进一步说明研究方向或补充筛选条件。
ATG9A is a critical transmembrane protein involved in autophagy, a cellular degradation pathway essential for maintaining homeostasis. As one of the few autophagy-related (ATG) proteins with a transmembrane domain, ATG9A plays a pivotal role in forming and mobilizing autophagosomal membranes. It shuttles between the trans-Golgi network, endosomes, and autophagosomes, facilitating lipid transfer and membrane expansion during autophagosome biogenesis. Dysregulation of ATG9A has been linked to neurodegenerative diseases, cancer, and metabolic disorders, highlighting its biological significance.
ATG9A antibodies are indispensable tools for studying autophagy mechanisms. They enable the detection and localization of endogenous ATG9A via techniques like Western blotting (WB), immunofluorescence (IF), and immunoprecipitation (IP). These antibodies often target specific epitopes, such as the N-terminal or C-terminal regions, to assess protein expression, post-translational modifications, or interactions with other autophagy regulators. Commercial ATG9A antibodies are typically validated in knockout cell lines to ensure specificity, as cross-reactivity with homologous proteins (e.g., ATG9B) can occur.
Research using ATG9A antibodies has advanced our understanding of its role in physiological processes like nutrient sensing, organelle quality control, and pathogen clearance. Additionally, these reagents aid in exploring therapeutic strategies targeting autophagy in diseases such as Alzheimer’s and Parkinson’s. However, variability in antibody performance across experimental conditions necessitates careful optimization and validation in each study.
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