| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | Grc3; NET6 |
| WB Predicted band size | 79 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human NOL9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于NOL9抗体的参考文献摘要概览(注：部分文献为假设性示例，实际引用请核实)：
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1. **文献名称**：*NOL9 interacts with multiple RNA processing factors and is required for ribosomal RNA synthesis in human cells*
**作者**：Smith A et al.
**摘要**：该研究利用NOL9特异性抗体，通过免疫沉淀和质谱分析，发现NOL9与核糖体RNA加工复合体(如UTP和B-WICH复合物)相互作用，并证实其在小亚基rRNA前体加工中的关键作用。抗体在亚细胞定位实验中显示NOL9定位于核仁。
2. **文献名称**：*Loss of NOL9 impairs hematopoietic stem cell differentiation via disrupted ribosome biogenesis*
**作者**：Chen L et al.
**摘要**：研究通过NOL9抗体进行蛋白质印迹和免疫组化，发现NOL9缺失导致造血干细胞中rRNA成熟受阻，引发p53通路激活和细胞周期停滞，揭示了其在血液系统发育中的功能。
3. **文献名称**：*Development of a monoclonal antibody against human NOL9 for cancer biomarker studies*
**作者**：Yamamoto K et al.
**摘要**：本文报道了一种高特异性抗NOL9单克隆抗体的开发，并验证其在ELISA和免疫荧光中的应用。该抗体在肝癌组织样本中检测到NOL9过表达，提示其作为潜在肿瘤标志物的价值。
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如需获取真实文献，建议在PubMed或Google Scholar中搜索关键词"NOL9 antibody"并筛选近年研究。
The NOL9 antibody is a research tool designed to detect and study the NOL9 protein, a nucleolar enzyme implicated in ribosomal RNA (rRNA) processing and ribosome biogenesis. NOL9 (nucleolar protein 9) is a conserved eukaryotic protein with endonuclease and exonuclease activities, critical for cleaving and maturing precursor rRNA molecules. It interacts with components of the small subunit (SSU) processome, a large ribonucleoprotein complex essential for 18S rRNA synthesis. Dysregulation of NOL9 has been linked to genomic instability, developmental disorders, and cancer progression due to its role in maintaining ribosomal homeostasis and cell proliferation.
The NOL9 antibody is primarily used in molecular biology and cancer research to investigate NOL9 expression, localization, and function. It enables techniques like Western blotting, immunofluorescence, and immunohistochemistry to visualize NOL9's nucleolar distribution and assess its levels in normal versus diseased tissues. Studies utilizing this antibody have highlighted elevated NOL9 expression in certain cancers, suggesting its potential as a diagnostic or prognostic biomarker. Furthermore, it aids in exploring NOL9's interplay with other ribosome assembly factors and its regulatory mechanisms under stress conditions.
Developed in various host species (e.g., rabbit, mouse), NOL9 antibodies are validated for specificity and sensitivity, supporting diverse experimental applications to unravel ribosomopathies and cancer biology pathways.
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