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产品描述：山羊三碘甲状腺原氨酸(T3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的T3含量。试剂盒检测范围为0.5 ng/mL-8 ng/mL，灵敏度为0.5 ng/mL。适用于科研领域如畜牧动物生理研究、饲料营养干预实验、甲状腺相关疾病机制探索及药物代谢评估等方向，有助于研究山羊内分泌功能、代谢疾病模型或环境因素对甲状腺轴的影响本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：T3
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.5 ng/mL-8 ng/mL
灵敏度：0.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat T3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 90-103 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 85-102 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 90-97 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 88-99 | ||||||
回收率：
| The recovery of goat T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 86-102 | |||||
| EDTA plasma (n=4) | 96 | 92-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 80.1660.1680.167 40.2810.2880.285 20.5580.5530.556 11.2621.2471.255 0.52.1052.1382.122 | |||||||
货期：3-5 working days
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