| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PIP4K2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于PIP4K2A抗体的参考文献及其摘要概括：
1. **"A role for PIP4K2A in neurodevelopmental disorders and epilepsy"**
- **作者**: Li, X. et al.
- **摘要**: 本研究利用特异性PIP4K2A抗体检测其在神经元中的表达，发现PIP4K2A缺失导致突触囊泡运输异常，与自闭症和癫痫相关。抗体通过免疫印迹和免疫组化验证，支持其在神经发育中的作用。
2. **"Development of a monoclonal antibody targeting PIP4K2A for cancer biomarker discovery"**
- **作者**: Chen, Y. & Wang, L.
- **摘要**: 报道了一种新型单克隆抗体的开发，该抗体针对PIP4K2A的C端结构域，用于检测多种癌症组织中的蛋白水平升高。研究通过ELISA和免疫荧光证实其高特异性，提示PIP4K2A可能作为肿瘤诊断标志物。
3. **"PIP4K2A regulates insulin signaling through antibody-validated protein interaction networks"**
- **作者**: Gupta, R. et al.
- **摘要**: 使用PIP4K2A抗体进行免疫共沉淀实验，揭示了其与胰岛素受体底物的相互作用，阐明其在胰岛素信号通路中的调控机制。抗体特异性经siRNA敲低实验验证，为代谢疾病研究提供工具。
注：上述文献为虚拟示例，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词“PIP4K2A antibody”、“PIP4K2A immunohistochemistry”等查找具体研究。
**Background of PIP4K2A Antibody**
PIP4K2A (Phosphatidylinositol-5-Phosphate 4-Kinase Type 2 Alpha) is an enzyme encoded by the *PIP4K2A* gene, belonging to the phosphatidylinositol kinase family. It catalyzes the phosphorylation of phosphatidylinositol-5-phosphate (PI5P) to generate phosphatidylinositol-4.5-bisphosphate (PI4.5P2), a lipid secondary messenger involved in regulating cellular processes such as membrane trafficking, signal transduction, and cytoskeletal organization. PIP4K2A's activity impacts pathways linked to cell growth, metabolism, and stress responses, including the mTOR and AKT signaling networks.
Antibodies targeting PIP4K2A are essential tools for studying its expression, localization, and function in physiological and disease contexts. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to quantify protein levels or assess tissue-specific distribution. Research has implicated PIP4K2A in cancer, neurodegenerative disorders, and immune regulation. For example, elevated PIP4K2A expression correlates with poor prognosis in certain cancers, while its inhibition shows potential in sensitizing cells to chemotherapeutic agents.
Recent studies also highlight PIP4K2A's role in metabolic diseases and aging, driven by its interaction with autophagy and insulin signaling pathways. The development of specific PIP4K2A antibodies has enabled the exploration of its therapeutic relevance, including the evaluation of small-molecule inhibitors in preclinical models. As a regulator of phosphoinositide homeostasis, PIP4K2A remains a focal point for understanding lipid-mediated cellular regulation and disease mechanisms.
×
