| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | DSPS; SNAT |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human AANAT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于AANAT抗体的代表性文献(信息基于模拟生成)：
1. **文献名称**：*"Production and validation of specific antibodies for detecting arylalkylamine N-acetyltransferase (AANAT) in rodent tissues"*
**作者**：Ganguly S. et al.
**摘要**：本研究报道了针对大鼠AANAT蛋白的多克隆抗体的制备，通过Western blot和免疫组化验证其特异性，证实其在松果体及视网膜组织中的昼夜表达差异。
2. **文献名称**：*"AANAT antibody reveals circadian regulation of melatonin synthesis in human pinealocytes"*
**作者**：Skene D.J. et al.
**摘要**：利用单克隆AANAT抗体，揭示了人松果体细胞中AANAT蛋白的昼夜节律性表达，并证明其活性与褪黑素分泌直接相关。
3. **文献名称**：*"Immunohistochemical localization of AANAT in mammalian retina: species-specific variations"*
**作者**：Tosini G. et al.
**摘要**：通过比较多种哺乳动物视网膜中的AANAT分布，使用特异性抗体发现其在光敏感细胞中的定位存在物种差异，提示功能分化。
4. **文献名称**：*"AANAT antibody cross-reactivity in non-mammalian models: implications for evolutionary studies"*
**作者**：Falcón J. et al.
**摘要**：评估AANAT抗体在鱼类和鸟类中的交叉反应性，为比较生物学研究提供工具，并探讨其在进化保守性中的意义。
*注：以上文献为示例性质，实际引用需以具体数据库检索结果为准。*
**Background of AANAT Antibodies**
AANAT (arylalkylamine N-acetyltransferase) is a critical enzyme in melatonin synthesis, catalyzing the conversion of serotonin to N-acetylserotonin, the immediate precursor of melatonin. It plays a central role in regulating circadian rhythms, as its activity fluctuates rhythmically under control of the suprachiasmatic nucleus (SCN). AANAT antibodies are essential tools for studying the expression, localization, and function of this enzyme in various tissues, particularly the pineal gland, retina, and peripheral organs.
These antibodies are widely used in research applications such as Western blotting, immunohistochemistry, and ELISA to investigate AANAT's involvement in sleep-wake cycles, mood disorders, and cancer progression. Species-specific AANAT antibodies (e.g., human, mouse, rat) enable cross-species comparative studies, aiding in translational research. Dysregulation of AANAT has been linked to circadian disruptions, depression, and metabolic disorders, making its detection vital for understanding disease mechanisms.
Commercial AANAT antibodies are typically validated for specificity and sensitivity, often targeting conserved epitopes. Researchers also utilize them to explore pharmacological or environmental impacts on melatonin pathways. Their development continues to advance studies in chronobiology, neuroendocrinology, and therapeutic targeting of rhythm-related diseases.
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