| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | ASH2; Bre2; ASH2L1; ASH2L2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ASH2L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ASH2L抗体的3篇参考文献示例(文献信息为模拟虚构，仅供格式参考)：
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1. **文献名称**: *ASH2L regulates histone H3K4 methylation in leukemia stem cells*
**作者**: Smith J, et al.
**摘要**: 本研究利用ASH2L特异性抗体，通过ChIP-seq技术揭示了ASH2L在白血病干细胞中调控H3K4me3修饰的作用，证明其缺失导致分化阻滞和癌基因表达失调。
2. **文献名称**: *Structural basis of ASH2L in MLL complex assembly*
**作者**: Chen L, et al.
**摘要**: 通过免疫共沉淀结合ASH2L抗体，阐明了ASH2L与MLL复合体其他组件的相互作用机制，发现其C端结构域对维持复合体稳定性至关重要。
3. **文献名称**: *ASH2L promotes somatic cell reprogramming via epigenetic remodeling*
**作者**: Wang Y, et al.
**摘要**: 研究使用ASH2L抗体进行Western blot和免疫荧光染色，证实ASH2L通过增强多能性基因启动子区域的H3K4me3水平，促进体细胞重编程为诱导多能干细胞。
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*注：以上文献为示例，实际引用时需核实真实数据库(如PubMed)中的具体论文信息。*
The ASH2L (Absent, Small, or Homeotic-Like) antibody is a crucial tool for studying the ASH2L protein, a conserved core component of the multi-protein COMPASS (Complex of Proteins Associated with Set1) family. These complexes catalyze histone H3 lysine 4 methylation (H3K4me), a key epigenetic modification associated with active gene transcription. ASH2L stabilizes the COMPASS complex by interacting with other core subunits like WDR5. RbBP5. and DPY30. and its presence is essential for maintaining global H3K4me3 levels. Dysregulation of ASH2L has been implicated in developmental disorders, cancers, and other diseases, making it a target for epigenetic research.
ASH2L antibodies are widely used in techniques such as Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to investigate its expression, localization, and interactions in cellular models or tissues. Commercial antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, and validated for species cross-reactivity (e.g., human, mouse, rat). Researchers rely on these antibodies to explore ASH2L's role in chromatin remodeling, stem cell differentiation, and oncogenesis. Proper validation via knockout controls or siRNA knockdown is critical to ensure specificity, given the structural similarities among COMPASS subunits. Overall, ASH2L antibodies are indispensable for dissecting the molecular mechanisms of epigenetic regulation in health and disease.
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