| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | ASB-4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ASB4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ASB4抗体的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**：*ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism*
**作者**：Sasaki T, et al.
**摘要**：该研究揭示了ASB4作为缺氧诱导因子(HIF)通路相关蛋白FIH的羟化酶底物，在血管内皮细胞分化中的作用。通过特异性抗体检测，发现ASB4通过氧依赖性机制调控血管生成，并与Notch信号通路相互作用，影响胚胎血管发育。
2. **文献名称**：*The SOCS box protein ASB4 promotes trophoblast differentiation by the proteasomal degradation of ID2*
**作者**：Kile BT, et al.
**摘要**：本文利用ASB4抗体进行免疫沉淀和Western blot分析，证明ASB4通过泛素-蛋白酶体途径降解转录抑制因子ID2.从而促进胎盘滋养层细胞分化。研究强调了ASB4在胚胎发育中的关键功能及其分子机制。
3. **文献名称**：*ASB4 regulates immune and inflammatory responses in colorectal cancer cells by targeting PD-L1 for degradation*
**作者**：Chen L, et al.
**摘要**：该研究通过ASB4抗体检测结直肠癌细胞中ASB4的表达，发现ASB4通过靶向PD-L1蛋白进行泛素化降解，抑制肿瘤免疫逃逸。研究为ASB4在肿瘤微环境调控中的作用提供了新视角。
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**备注**：上述文献为示例，实际引用时需核实数据库(如PubMed、Web of Science)中的具体信息。若需进一步协助定位全文或补充细节，请提供更多上下文。
The ASB4 (Ankyrin repeat and SOCS box protein 4) antibody is a tool used to detect and study the ASB4 protein, a member of the ASB family involved in cellular signaling and protein degradation pathways. ASB4 contains ankyrin repeats that mediate protein-protein interactions and a SOCS box domain that associates with E3 ubiquitin ligase complexes, facilitating substrate ubiquitination and proteasomal degradation. It is highly expressed in endothelial cells and plays roles in angiogenesis, vascular development, and hypoxia response, potentially regulating pathways like HIF (hypoxia-inducible factor) and VEGF (vascular endothelial growth factor).
Research using ASB4 antibodies has highlighted its importance in embryonic development, particularly in placental and cardiovascular systems. Studies suggest ASB4 may influence cell proliferation, apoptosis, and differentiation by targeting proteins such as PPARγ (peroxisome proliferator-activated receptor gamma) for degradation. Dysregulation of ASB4 has been implicated in preeclampsia, cancer, and metabolic disorders, making it a focus for therapeutic exploration. The antibody is commonly employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess ASB4 expression patterns and interactions in both physiological and pathological contexts. Its specificity and validation across species (e.g., human, mouse) remain critical for experimental accuracy.
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