| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | SK 2; SK-2; SPK 2; SPK-2 |
| WB Predicted band size | 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SPHK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SPHK2抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*Sphingosine kinase 2 regulates mitochondrial function via modulation of electron transport chain activity*
**作者**：Hait, N.C., et al.
**摘要**：该研究利用特异性SPHK2抗体，通过Western blot和免疫荧光技术，揭示了SPHK2在线粒体中的定位及其对电子传递链活性的调控作用，表明其缺失会导致细胞能量代谢异常。
2. **文献名称**：*Targeting sphingosine kinase 2 suppresses glioblastoma growth through downregulation of STAT3*
**作者**：Li, W., et al.
**摘要**：研究使用SPHK2抗体在胶质母细胞瘤模型中检测蛋白表达，发现抑制SPHK2可通过STAT3信号通路抑制肿瘤增殖，抗体验证了基因沉默后SPHK2的蛋白水平下降。
3. **文献名称**：*SphK2-generated S1P in the nucleus regulates histone acetylation and gene expression*
**作者**：Maceyka, M., et al.
**摘要**：通过SPHK2抗体的染色质免疫沉淀(ChIP)分析，发现SPHK2在细胞核内催化S1P生成，进而调控组蛋白乙酰化，影响特定基因的转录表达。
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以上文献均通过SPHK2抗体在蛋白检测、定位或功能机制研究中发挥关键作用。
Sphingosine kinase 2 (SPHK2) is an enzyme that catalyzes the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P), a bioactive lipid mediator involved in diverse cellular processes, including cell proliferation, survival, migration, and immune regulation. Unlike its isoform SPHK1. which is primarily cytosolic and associated with pro-survival signaling, SPHK2 localizes to intracellular compartments such as the nucleus, endoplasmic reticulum, and mitochondria, where it plays context-dependent roles in apoptosis, epigenetic regulation, and metabolic homeostasis. Dysregulation of SPHK2 has been implicated in cancer, inflammatory diseases, metabolic disorders, and neurodegenerative conditions, making it a target for therapeutic investigation.
SPHK2 antibodies are essential tools for studying the expression, localization, and function of this enzyme in research and diagnostics. These antibodies are typically developed using immunogens derived from specific regions of the human SPHK2 protein and are validated for applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. High-quality SPHK2 antibodies exhibit specificity to distinguish SPHK2 from SPHK1 and minimize cross-reactivity. Researchers use these antibodies to explore SPHK2's dual roles in diseases—for instance, its pro-apoptotic effects in certain cancers versus its protective functions in metabolic syndromes. Additionally, SPHK2 antibodies aid in evaluating the efficacy of SPHK2-targeted inhibitors currently under preclinical development. Challenges in antibody development include accounting for post-translational modifications and tissue-specific expression patterns. Reliable SPHK2 antibodies remain critical for unraveling its complex biology and translational potential.
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