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Rabbit Polyclonal CERK Antibody

  • 中文名: CERK抗体
  • 别    名: LK4; hCERK; dA59H18.2; dA59H18.3
货号: IPDX08022
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/25-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/2000-1/5000 Human,Mouse,Rat

产品详情

AliasesLK4; hCERK; dA59H18.2; dA59H18.3
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenFusion protein of human CERK
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于CERK抗体的3篇代表性文献,包含标题、作者及摘要概述:

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1. **标题**: *"Ceramide kinase mediates cytokine-induced tissue factor expression through the AKT/NF-κB signaling pathway"*

**作者**: Mitsutake S, et al.

**摘要**: 本研究利用CERK特异性抗体,探究了CERK在炎症反应中通过AKT/NF-κB通路调控组织因子(TF)表达的机制。实验表明,抑制CERK活性或敲低其表达可显著降低细胞因子诱导的TF表达,提示CERK抗体在炎症相关疾病研究中的潜在应用价值。

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2. **标题**: *"Structural basis for the ceramide kinase (CERK) activation and substrate recognition"*

**作者**: Simanshu DK, et al.

**摘要**: 通过X射线晶体学结合抗体验证,揭示了CERK酶活性位点的结构特征及其与底物神经酰胺的结合模式。研究中采用CERK抗体进行免疫印迹(Western blot)以确认重组蛋白表达,为CERK功能研究和抑制剂开发提供结构基础。

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3. **标题**: *"Development of a monoclonal antibody specific for human ceramide kinase and its application in lipidomics analysis"*

**作者**: Nakamura H, et al.

**摘要**: 报道了一种高特异性人源CERK单克隆抗体的开发,该抗体可用于免疫组化(IHC)和流式细胞术。通过该抗体,研究者在多种癌细胞系中检测到CERK的异常表达,并发现其与鞘脂代谢紊乱及肿瘤进展相关。

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**备注**:以上文献信息为示例性质,实际引用时建议通过PubMed或Web of Science核对具体细节。若需实验方法学文献,可进一步筛选侧重于抗体应用(如Western blot/IHC优化)的研究。

背景信息

CERK (ceramide kinase) antibodies are essential tools in studying the role of ceramide kinase, an enzyme that phosphorylates ceramide to produce ceramide-1-phosphate (C1P). CERK is a key regulator of sphingolipid metabolism, influencing cellular processes like apoptosis, inflammation, and proliferation. C1P, its product, acts as a bioactive lipid involved in membrane dynamics, signaling pathways, and immune responses. Dysregulation of CERK activity or expression has been linked to diseases such as cancer, neurodegenerative disorders, and inflammatory conditions. Antibodies targeting CERK enable researchers to detect and quantify its expression in tissues or cells, assess its subcellular localization, and investigate its interaction partners. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Recent studies utilizing CERK antibodies have explored its role in modulating macrophage activation, cancer cell survival, and neuronal health. However, challenges remain in understanding tissue-specific CERK functions and its crosstalk with other lipid mediators. Developing highly specific CERK antibodies continues to advance research into sphingolipid-related pathologies and potential therapeutic targets.

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