| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ANF; ANP; PND; ATFB6; CDD-ANF |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NPPA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NPPA抗体的3篇参考文献及其简要概括：
1. **《Development of a High-Sensitivity ELISA for Human ANP Using Novel Monoclonal Antibodies》**
- 作者：Smith A, et al.
- 摘要：研究团队开发了一种基于新型NPPA单克隆抗体的高灵敏度ELISA检测方法，用于定量人血浆中的ANP水平，为心力衰竭的早期诊断提供新工具。
2. **《NPPA Gene Expression and Antibody-Based Detection in Hypertensive Rat Models》**
- 作者：Johnson R, et al.
- 摘要：通过免疫组化和Western blot分析NPPA蛋白在高血压大鼠心脏中的表达，揭示了ANP抗体在病理机制研究中的应用价值，表明ANP水平与血压调节相关。
3. **《Clinical Utility of NPPA Autoantibodies as a Biomarker in Cardiovascular Disease Prognosis》**
- 作者：Lee S, et al.
- 摘要：探讨了血清中NPPA自身抗体的存在与慢性心力衰竭患者预后的关联，提出其可作为心血管疾病风险分层的潜在生物标志物。
(注：以上文献信息为模拟示例，实际引用需查询具体数据库。)
**Background of NPPA Antibody**
The NPPA (Natriuretic Peptide Precursor A) gene encodes atrial natriuretic peptide (ANP), a hormone primarily secreted by cardiac atrial cells in response to mechanical stress, volume overload, or hypertension. ANP plays a critical role in regulating blood pressure, fluid balance, and cardiovascular homeostasis by promoting natriuresis, diuresis, and vasodilation. Dysregulation of ANP expression is associated with cardiovascular diseases, including heart failure, atrial fibrillation, and hypertension.
NPPA antibodies are essential tools for detecting and quantifying ANP in research and diagnostics. These antibodies target specific epitopes of the ANP precursor or mature peptide, enabling applications such as immunohistochemistry, Western blotting, ELISA, and immunofluorescence. They aid in studying ANP's physiological roles, its secretion dynamics, and its pathological alterations in disease models.
The development of high-specificity NPPA antibodies has advanced understanding of ANP's interaction with receptors (e.g., NPR-A) and its downstream signaling pathways. Cross-reactivity studies often confirm their applicability across species (e.g., human, mouse, rat), supporting translational research. Commercial NPPA antibodies are validated for precision, though variability in sensitivity or specificity between clones requires careful experimental optimization. Overall, NPPA antibodies remain pivotal in cardiovascular research, biomarker discovery, and therapeutic targeting.
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