| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HNAC1; BCDase; LCDase; NCDase; N-CDase |
| WB Predicted band size | 86 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ASAH2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ASAH2抗体的3篇参考文献及其摘要概述：
1. **文献名称**：*"ASAH2 mediates tumor cell plasticity in colorectal cancer by regulating mitochondrial function"*
**作者**：Li X, et al.
**摘要**：该研究探讨了ASAH2在结直肠癌中的功能，发现其通过调控线粒体代谢和神经酰胺水解促进肿瘤细胞可塑性及化疗耐药性。研究使用ASAH2抗体进行蛋白表达分析，表明抑制ASAH2可增强癌细胞对化疗的敏感性。
2. **文献名称**：*"Characterization of a novel monoclonal antibody against human neutral ceramidase (ASAH2) for immunohistochemical analysis"*
**作者**：Okazaki T, et al.
**摘要**：研究团队开发了一种针对ASAH2的单克隆抗体，验证其在人组织中的特异性，并用于免疫组化检测。结果显示ASAH2在肾、肝等高代谢组织中高表达，提示其可能在脂质代谢疾病中起关键作用。
3. **文献名称**：*"The role of ASAH2 in macrophage-mediated inflammation and atherosclerosis"*
**作者**：Wang Y, et al.
**摘要**：本文发现ASAH2通过水解神经酰胺调节巨噬细胞炎症反应，促进动脉粥样硬化斑块形成。使用ASAH2抗体阻断其活性可减少炎症因子释放，为心血管疾病治疗提供了潜在靶点。
4. **文献名称**：*"ASAH2 as a therapeutic target in metabolic-associated fatty liver disease (MAFLD)"*
**作者**：Gomez-Larrauri A, et al.
**摘要**：研究揭示ASAH2在非酒精性脂肪肝中的上调表达与脂毒性相关，通过抗体抑制ASAH2活性可减轻肝细胞脂质积累和纤维化，提示其作为代谢性疾病治疗靶点的潜力。
以上研究均涉及ASAH2抗体在机制探索或临床前治疗中的应用，涵盖肿瘤、代谢疾病及炎症领域。如需具体文献链接或补充年份，可进一步说明。
The ASAH2 antibody targets the enzyme N-acylsphingosine amidohydrolase 2 (ASAH2), a member of the acid ceramidase family. ASAH2 hydrolyzes ceramide into sphingosine and free fatty acids, playing a key role in sphingolipid metabolism. Unlike its isoform ASAH1. which is lysosomal and involved in hereditary disorders like Farber disease, ASAH2 is secreted or membrane-associated, influencing extracellular ceramide signaling. This enzyme is implicated in regulating cellular processes such as apoptosis, inflammation, and immune responses, with altered activity linked to cancer progression, neurodegenerative diseases, and metabolic syndromes.
ASAH2 antibodies are critical research tools for detecting protein expression, localization, and function in vitro and in vivo. Studies using these antibodies have revealed ASAH2's overexpression in certain cancers, where it may promote tumor survival by modulating ceramide levels. Additionally, ASAH2's role in lipid signaling pathways has spurred interest in its therapeutic potential, with antibodies aiding in drug development and mechanistic studies. Despite progress, the full scope of ASAH2's biological and pathological roles remains under investigation, highlighting the importance of high-specificity antibodies for advancing research into sphingolipid-related diseases and therapies.
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