| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| WB Predicted band size | 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CAT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与CAT(过氧化氢酶)抗体相关的参考文献及其摘要概括：
1. **文献名称**：*"Development and characterization of a novel monoclonal antibody against human catalase"*
**作者**：Smith A, et al.
**摘要**：研究团队开发了一种高特异性抗人过氧化氢酶单克隆抗体，通过免疫小鼠和杂交瘤技术筛选获得。该抗体在Western blot和免疫组化中表现出高灵敏度和低交叉反应性，适用于氧化应激相关疾病的分子机制研究。
2. **文献名称**：*"Catalase antibody-based detection of oxidative stress in neurodegenerative diseases"*
**作者**：Chen L, et al.
**摘要**：文章利用抗CAT抗体检测阿尔茨海默病和帕金森病患者脑组织中的过氧化氢酶表达水平，发现其与氧化损伤标志物呈负相关，提示CAT活性降低可能加剧神经退行性病变。
3. **文献名称**：*"A comparative study of commercial catalase antibodies for diagnostic applications"*
**作者**：Rodriguez M, et al.
**摘要**：研究对比了市售多种CAT抗体的性能，评估其在ELISA、免疫荧光等平台的特异性和稳定性，为临床诊断和基础研究中选择合适试剂提供参考依据。
(注：以上文献为示例性内容，实际引用时需核实真实文献信息。)
CAT antibodies, short for Choline Acetyltransferase antibodies, are immunological tools targeting the enzyme choline acetyltransferase (ChAT). ChAT is critical for synthesizing the neurotransmitter acetylcholine (ACh) by catalyzing the transfer of an acetyl group from acetyl-CoA to choline. This enzyme is predominantly expressed in cholinergic neurons of the central and peripheral nervous systems, playing essential roles in motor control, memory, and cognitive functions. CAT antibodies are widely used in neuroscience research to identify and study cholinergic neurons, track their projections, and investigate pathologies linked to ACh dysfunction, such as Alzheimer’s disease, Parkinson’s disease, and myasthenic syndromes.
These antibodies are typically produced in hosts like rabbits or mice using purified ChAT protein or peptide fragments as immunogens. They are validated for applications including immunohistochemistry (IHC), Western blotting, and immunofluorescence, enabling visualization of ChAT distribution in tissues or cultured cells. Specificity is confirmed through knockout controls or siRNA-mediated ChAT suppression. CAT antibodies have also facilitated the discovery of non-neuronal ChAT expression, such as in immune cells or epithelial tissues, broadening understanding of ACh's roles beyond neurotransmission. Despite their utility, variability in antibody performance across species or fixation methods necessitates careful validation for experimental consistency. Overall, CAT antibodies remain indispensable for dissecting cholinergic system biology and its implications in health and disease.
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