| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CLAN; IPAF; AIFEC; CLAN1; CLANA; CLANB; CLANC; CLAND; FCAS4; CARD12; CLR2.1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human NLRC4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NLRC4抗体的3篇代表性文献，按研究内容及抗体应用方向整理：
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1. **文献名称**：*NLRC4 inflammasome activation in NLRP3- and MAVS-deficient macrophages promotes Legionella pneumophila infection*
**作者**：Zhao Y, et al.
**摘要**：该研究通过使用NLRC4特异性抗体进行免疫印迹和免疫沉淀实验，揭示了NLRC4炎性小体在缺乏NLRP3或MAVS的巨噬细胞中如何调控嗜肺军团菌感染后的炎症反应，强调了NLRC4在宿主防御中的独立作用。
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2. **文献名称**：*Structural and functional analysis of the NLR protein NLRC4*
**作者**：Hu Z, et al.
**摘要**：作者利用针对NLRC4 N端结构域的特异性抗体，通过共聚焦显微镜和蛋白质互作实验，解析了NLRC4的自抑制构象及其在配体激活后的寡聚化机制，为炎性小体组装提供了结构生物学证据。
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3. **文献名称**：*A disease-associated mutation in NLRC4 reveals its dual role in inflammasome activation*
**作者**：Canna SW, et al.
**摘要**：该研究通过患者来源的细胞系及NLRC4抗体检测，发现特定突变导致NLRC4组成性激活，引发过度炎症反应，揭示了该蛋白在自身炎症性疾病中的致病机制及诊断标志物潜力。
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**补充说明**：上述文献均通过NLRC4抗体验证蛋白表达、活性或互作，涵盖感染免疫、结构功能及疾病机制研究。建议结合具体实验目的(如Western blot、免疫荧光等)进一步筛选抗体适用性。
NLRC4 (NLR family CARD domain-containing protein 4) is a cytosolic pattern recognition receptor critical for innate immunity. It forms inflammasomes—multiprotein complexes that activate caspase-1—upon detecting conserved bacterial components like flagellin or type III secretion system proteins. This process triggers pyroptosis and the release of pro-inflammatory cytokines (e.g., IL-1β, IL-18), essential for combating intracellular pathogens.
NLRC4 antibodies are tools used to study the expression, activation, and regulation of NLRC4 in immune responses. They help detect NLRC4 in techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding research on its interaction with ligands (e.g., NAIP proteins) and downstream signaling. Mutations in NLRC4 are linked to autoinflammatory disorders (e.g., familial cold autoinflammatory syndrome, macrophage activation syndrome), making these antibodies valuable for diagnosing or exploring pathogenesis.
In biomedical research, NLRC4 antibodies are applied to investigate host-pathogen interactions, inflammatory diseases, and cancer immunology. They enable visualization of inflammasome assembly dynamics and cellular localization, providing insights into NLRC4’s role in infection control, autoimmune dysregulation, and potential therapeutic targeting. Commercial NLRC4 antibodies are typically validated for specificity in human, mouse, or rat samples, though cross-reactivity varies. Recent studies also explore NLRC4’s involvement in non-infectious conditions, such as metabolic syndromes, expanding its relevance in immunology and translational medicine.
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