WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/200 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
Aliases | Gsg3; CAPPA3 |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | Fusion protein of human CAPZA3 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CAPZA3抗体的模拟参考文献示例(请注意文献信息为虚构,建议通过学术数据库核实真实文献):
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1. **文献名称**: "CAPZA3 is essential for spermatogenesis in mice: Characterization of a testis-specific actin-capping protein"
**作者**: Zhang, Y., et al.
**摘要**: 本研究利用特异性CAPZA3抗体,发现该蛋白在小鼠睾丸生精细胞中高表达,尤其在精子形成阶段。基因敲除实验表明CAPZA3缺失导致精子形态异常和不育,提示其在F-actin动态调控中的关键作用。
2. **文献名称**: "Development of a polyclonal antibody against human CAPZA3 and its application in male infertility studies"
**作者**: Chen, L., et al.
**摘要**: 研究团队开发了人源CAPZA3多克隆抗体,并通过Western blot和免疫组化验证其特异性。该抗体用于分析不育患者睾丸活检样本,发现CAPZA3表达水平降低与精子发生障碍显著相关。
3. **文献名称**: "CAPZA3 as a potential biomarker for testicular germ cell tumors: Immunohistochemical analysis using a novel monoclonal antibody"
**作者**: Li, X., et al.
**摘要**: 通过构建CAPZA3单克隆抗体,研究者发现其在睾丸生殖细胞肿瘤中异常高表达。抗体特异性经流式细胞术验证,提示CAPZA3可能作为诊断标记物或治疗靶点。
4. **文献名称**: "Dynamic localization of CAPZA3 during acrosome formation revealed by antibody-based imaging"
**作者**: García-Robledo, J.E., et al.
**摘要**: 利用免疫荧光和CAPZA3特异性抗体,研究揭示了该蛋白在精子顶体形成过程中的时空分布变化,表明其与actin重塑密切关联,为生殖细胞发育机制提供新见解。
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建议通过**PubMed**或**Web of Science**搜索真实文献,关键词:CAPZA3 antibody, spermatogenesis, actin capping。
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