| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CAPZ; CAPPA2 |
| WB Predicted band size | 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CAPZA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CAPZA2抗体的3篇参考文献及简要摘要：
1. **标题**：*CAPZA2 modulates cell migration and invasion in breast cancer via actin cytoskeleton dynamics*
**作者**：Li X, et al.
**摘要**：该研究通过免疫荧光和Western blot分析CAPZA2在乳腺癌中的表达，发现其通过调节F-actin组装影响细胞迁移和侵袭能力，提示其作为转移抑制因子的潜在作用。
2. **标题**：*CAPZA2 interacts with neurodevelopmental proteins and regulates dendritic spine formation*
**作者**：Wang Y, et al.
**摘要**：利用CAPZA2特异性抗体进行共免疫沉淀和质谱分析，揭示了CAPZA2与突触相关蛋白的相互作用，表明其在神经元树突棘形态发生中的关键调控功能。
3. **标题**：*Proteomic analysis of CAPZA2-deficient cells reveals its role in autophagosome-lysosome fusion*
**作者**：Chen H, et al.
**摘要**：通过敲低CAPZA2并结合免疫印迹和免疫电镜技术，发现其缺失导致自噬体-溶酶体融合障碍，提示CAPZA2通过稳定微丝网络参与自噬调控。
注：以上文献为示例，实际引用需核对PubMed/Google Scholar等数据库获取真实论文信息。
The CAPZA2 antibody targets the alpha-2 subunit of the F-actin capping protein (CapZ), a conserved heterodimer (α/β) crucial for regulating actin filament dynamics. CapZ binds to the fast-growing (barbed) ends of actin filaments, stabilizing them by preventing uncontrolled polymerization or depolymerization. This regulation is vital for processes like cell motility, morphogenesis, and endocytosis. The CAPZA2 subunit, encoded by the CAPZA2 gene, is ubiquitously expressed and interacts with membrane-associated proteins, signaling molecules, and other cytoskeletal regulators (e.g., ARP2/3 complex, cofilin) to coordinate actin remodeling.
Antibodies against CAPZA2 are widely used in research to study its localization, expression levels, and functional roles in cellular processes. Dysregulation of CAPZA2 has been linked to pathologies, including cancer metastasis (e.g., breast, prostate) and neurological disorders, where aberrant actin dynamics contribute to disease progression. Commercial CAPZA2 antibodies are typically validated for techniques like Western blotting, immunofluorescence, and immunohistochemistry. Recent studies also explore its involvement in immune cell function and synaptic plasticity, highlighting its broad relevance. However, specificity validation (e.g., knockout controls) remains essential due to potential cross-reactivity with other CapZ isoforms.
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