| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | BS-17 |
| WB Predicted band size | 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CAST |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CAST(Calpastatin)抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Calpastatin Antibody Reveals Tissue-Specific Expression Patterns in Neurodegenerative Disorders*
**作者**：Smith J., et al.
**摘要**：研究利用特异性CAST抗体分析阿尔茨海默病模型小鼠脑组织，发现calpastatin表达水平显著下降，提示其与神经元钙蛋白酶活性异常及tau蛋白过度磷酸化相关。
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2. **文献名称**：*Autoantibodies Targeting Calpastatin in Rheumatoid Arthritis: A Novel Biomarker*
**作者**：Li Y., et al.
**摘要**：通过ELISA和免疫印迹法，发现类风湿性关节炎患者血清中存在抗CAST自身抗体，可能与炎症信号通路异常激活及关节损伤相关，提示其作为潜在诊断标志物。
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3. **文献名称**：*Prognostic Value of Calpastatin in Colorectal Cancer: An Immunohistochemical Study*
**作者**：Garcia R., et al.
**摘要**：使用CAST抗体对结肠癌组织进行免疫组化分析，发现高表达calpastatin的患者总生存期延长，表明其可能通过抑制钙蛋白酶依赖性凋亡通路抑制肿瘤转移。
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*注：上述文献为示例，实际引用时需核对真实存在的论文及作者信息。建议通过PubMed或Google Scholar以关键词“Calpastatin antibody”或“CAST antibody”检索最新研究。*
**Background of CAST Antibodies**
Calpastatin (CAST) is a endogenous inhibitor of calpains, a family of calcium-dependent cysteine proteases involved in diverse cellular processes, including apoptosis, cell cycle regulation, and cytoskeletal remodeling. CAST binds to calpains, blocking their proteolytic activity and maintaining cellular homeostasis. Dysregulation of the calpain-CAST system has been implicated in neurodegenerative diseases, muscular disorders, and cancer, making CAST a critical focus in biomedical research.
CAST antibodies are immunological tools developed to detect and quantify CAST expression in various biological samples. These antibodies are typically generated using recombinant CAST proteins or synthetic peptides as immunogens, enabling high specificity for either conserved or isoform-specific regions. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study CAST localization, expression levels, and interactions under physiological or pathological conditions.
Research applications include investigating CAST's role in neuroprotection, muscle atrophy, and tumor progression. For instance, altered CAST expression in Alzheimer’s disease models highlights its potential therapeutic relevance. Commercial CAST antibodies are often validated across species (human, mouse, rat) and tested for cross-reactivity to ensure reliability. As calpain-CAST imbalance emerges as a biomarker or therapeutic target, these antibodies remain vital for elucidating molecular mechanisms and developing diagnostic or therapeutic strategies.
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