| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1 |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PPP3CA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPP3CA抗体的3篇参考文献示例(文献内容为虚构，仅供参考格式)：
1. **文献名称**：*PPP3CA Expression in Breast Cancer: Correlation with Clinical Outcomes*
**作者**：Smith A, et al.
**摘要**：本研究通过Western blot和免疫组化技术，利用PPP3CA特异性抗体检测乳腺癌组织中PPP3CA蛋白的表达水平，发现其表达下调与患者不良预后显著相关，提示PPP3CA可能作为潜在的肿瘤抑制因子。
2. **文献名称**：*Localization of PPP3CA in Neuronal Synapses via Immunofluorescence*
**作者**：Lee B, et al.
**摘要**：通过免疫荧光技术结合PPP3CA抗体，揭示了PPP3CA蛋白在小鼠海马神经元突触中的特异性定位，并证明其在钙信号介导的突触可塑性中起关键作用。
3. **文献名称**：*PPP3CA-Mediated Calcineurin Signaling in Cardiac Hypertrophy*
**作者**：Garcia C, et al.
**摘要**：利用PPP3CA抗体进行免疫沉淀和Western blot分析，发现心脏肥大小鼠模型中PPP3CA蛋白活性显著升高，并通过调控NFAT通路促进病理性心肌重构。
**注**：以上文献为示例，实际引用时请通过PubMed或Google Scholar搜索真实文献(关键词：PPP3CA antibody, calcineurin A antibody, Western blot/immunofluorescence)。
PPP3CA, also known as calcineurin A α, is a catalytic subunit of the calcium/calmodulin-dependent protein phosphatase 2B (calcineurin), a serine/threonine phosphatase critical for calcium-mediated signaling pathways. Encoded by the PPP3CA gene, this enzyme regulates diverse cellular processes, including T-cell activation, neuronal development, and cardiac hypertrophy, primarily by dephosphorylating substrates like NFAT (nuclear factor of activated T-cells) transcription factors. PPP3CA antibodies are essential tools for studying calcineurin's role in immune response, synaptic plasticity, and disease mechanisms such as autoimmune disorders, cardiovascular diseases, and neurodegenerative conditions.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect PPP3CA expression, localization, and interaction partners in tissues or cultured cells. Due to calcineurin's heteromeric structure (a catalytic subunit paired with regulatory subunit PPP3R1/R2), specific PPP3CA antibodies help distinguish isoform-specific functions. Commercially available clones vary in host species (rabbit, mouse) and epitope targets, requiring validation for cross-reactivity with homologous subunits (e.g., PPP3CB or PPP3CC). Dysregulation of PPP3CA has been implicated in cancer progression and drug resistance (e.g., tacrolimus sensitivity), making its antibodies valuable for translational research. Researchers should optimize protocols based on sample type and consider post-translational modifications (e.g., autoinhibitory domain interactions) that may affect antibody binding.
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