| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | BUB1A; BUB1L; hBUB1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human BUB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于BUB1抗体的3篇参考文献，简要总结其研究内容：
1. **文献名称**：*BUB1 mediates cell death in response to chromosome missegregation and acts as a tumor suppressor*
**作者**：Ricke, R.M. et al. (2007)
**摘要**：研究利用BUB1特异性抗体，通过免疫印迹和免疫荧光技术，揭示BUB1在有丝分裂错误时触发细胞凋亡的机制，并证明其作为肿瘤抑制因子的潜在作用。
2. **文献名称**：*Overexpression of BUB1B contributes to progression of prostate cancer and predicts poor outcome in patients with prostate cancer*
**作者**：Yuan, B. et al. (2013)
**摘要**：通过免疫组化(使用BUB1抗体)分析前列腺癌组织样本，发现BUB1B蛋白高表达与肿瘤侵袭性及患者预后不良显著相关，提示其作为癌症生物标志物的潜力。
3. **文献名称**：*BUB1 regulates APC/C activity during mitosis*
**作者**：Tang, Z. et al. (2004)
**摘要**：利用BUB1抗体进行免疫沉淀和功能抑制实验，阐明BUB1通过调控APC/C复合物活性维持染色体分离准确性，为理解有丝分裂检查点机制提供关键证据。
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以上研究均涉及BUB1抗体的实验应用(如Western blot、免疫荧光、免疫组化)，聚焦于BUB1在癌症、基因组稳定性及细胞周期调控中的功能。
BUB1 (Budding Uninhibited by Benzimidazoles 1) is a conserved serine/threonine kinase critical for mitotic checkpoint control and chromosome segregation during cell division. As a key component of the spindle assembly checkpoint (SAC), BUB1 ensures accurate chromosome-microtubule attachment by monitoring kinetochore tension and recruiting other checkpoint proteins (e.g., BUBR1. MAD2) to delay anaphase until errors are corrected. Structurally, BUB1 contains an N-terminal KEN box for checkpoint signaling and a C-terminal kinase domain essential for its regulatory functions.
BUB1 antibodies are widely used tools in cell biology and cancer research to study mitotic fidelity, genomic instability, and SAC mechanisms. These antibodies enable detection of BUB1 expression levels, localization (e.g., at kinetochores during prometaphase), and phosphorylation status via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Dysregulation of BUB1 is linked to aneuploidy, tumor progression, and poor prognosis in cancers such as breast, lung, and colorectal carcinomas. Consequently, BUB1 antibodies also serve as potential biomarkers for assessing cancer aggressiveness or therapeutic responses.
Recent studies explore BUB1 inhibitors as anticancer agents targeting SAC-dependent cell survival. However, BUB1's dual roles in checkpoint signaling and chromosome alignment necessitate careful interpretation of experimental results. Validated BUB1 antibodies with high specificity remain crucial for elucidating its complex functions in mitosis and disease contexts.
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